Research Journal of Pharmacy and Technology

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Genetic study on Bacillus cereus


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1 Mosul University, College of Sciences, Biology Department, Iraq
     

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In this study, gram positive bacteria were isolated from contaminated soil in the Mosul city. Gram stain and biochemical tests were performed, which proved that the isolated bacterium was Bacillus cereus. The minimum inhibitory concentrations (MIC) of sodium chromate and sodium dodecyl sulfate (SDS) were determined to be 50 mM and 30,000μg/ml, respectively. Plasmid curing of isolated bacteria was also carried out with SDS in four sub lethal concentrations (100,200,600,1000) μg/ml, this process proved that the SDS is very efficient at 90% percentage of isolates of bacteria. The existence of plasmid was proved through plasmid extraction followed by electrophoresis. nitrous acid mutagenesis also was carried out on four intervals (15,30,45, and 60 minutes), 45 minutes was better other period on its effect on strains in their mutagenicity from chromate resistant to the chromate sensitive.

Keywords

Bacillus cereus, Plasmid, Chromate, Nitrous Acid, Resistance, SDS.
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  • Lapidus, A, Goltsman E, Auger S. Galleron N, Ségurens B, Dossat C, Land ML, Broussolle V, Brillard J, Guinebretiere MH, Sanchis V, Nguen-The C, Lereclus D, Richardson P, Wincker P, Weissenbach J, Ehrlich SD and Sorokin A. Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity.Chem Biol Interact.2008;171(2):236-49.

  • Okshevsky M, Louw MG, Lamela EO, Nilsson M, Tolker-Nielsen T and Meyr RL. A mutant transposon library of Bacillus cereus ATCC10987 reveals novel genes required for biofilm formation and implicates motility as an important factor for pellicle -biofilm formation. Microbiol. open. 2017; doi:10.1002/mbo3-552.

  • Wong H-C, Chang M-H and Fan J-Y. Incidence and characterization of Bacillus cereus isolates contaminating dairy products. Appl. Environ. Microbiol.1988;54:699-702.

  • Becker H, Schaller G, Wiese W and Terplan G. Bacillus cereus in infant foods and dried milk products. Int. J. Food Microbiol.1994;23:1-15.

  • Bottone EJ. Bacillus cereus, a volatile human pathogen. Clin Microbiol Rev.2010;23(2):382-98.

  • Granum PE and Lund T. Bacillus cereus and its food poisoning toxins. FEMS Microbiology Letters.1997; 157 :223-228.

  • Drobniewski FA. Bacillus cereus and related species. Clin Microbiol Rev.1993;6(4):324-38.

  • Trevors JT. Plasmid curing in bacteria. FEMS micrpbiol. revie.1986;32:57-99.

  • Bernhard K, Schrempf H and Goebel W. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J. Bacteriol.1978;133:897-903.

  • Macfaddin JFM. Biochemichal test for identification of medical bacteria. Williams and Wilkins, Inc., Baltimore, USA.1980.

  • Baron EJ, Peterson L and Fingold SM. Diagnostic microbiology. 9th ed., Mosby Year Book, Inc.,St.Louis,USA.1994.

  • Prescott LM, Harely TP and Klein DA.” Microbiology”.6th ed., McGraw-Hill Companies,Inc.,USA.2005.

  • Zamenhof S. Induction of mutations by deamination. Microbial Genetics Bull.1958; 16, 33.

  • Birnboin HC and Doly J. A rapid alkaline extraction procedure for screening recombination plasmid DNA. Nucleic Acid Res.1979;7:1513-1523.

  • Aabo S, Olsen JE, Threlfall EJ and Brown DJ. Characterization of non-virulence plasmids with homology to the virulence plasmid of Salmonella dublin. Res. Microbiol.1995;146:751-759.

  • Christensen JP, Olsen JE, Hansen HC and Bisgaard M. Characterization of Salmonella enterica serovar gallinarum Biovars gallinarum and Pullorum by plasmid profiling and biochemical analysis. Avian Pathol.1992; 21:461-470.

  • Fritze D. Taxonomy of the genus Bacillus and related genera: the aerobic endospore-forming bacteria. Phytopathol. 2004;94:1245-1248.

  • He M, Li X, Guo L, Miller SJ, Rensing C and Wang G. Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1.BMC Microbiology,2010;10:221.

  • Kannan SK, Lee KJ, Krishnamoorthy R, Purusothaman A Shanthi K and Rao R. Aerobic Chromate Reducing Bacillus cereus Isolated from the Heavy Metal Contaminated Ennore Creek Sediment, North of Chennai, Tamil Nadu, South East India. Res. Microbiol.2007;2:133-140.

  • Pal A and Paul AK. Aerobic chromate reduction by chromium –resistant bacteria isolated from serpentine soil. Microbiological Res. 2004; 159: 347-357.

  • Camargo FAO, Bento FM, Okeke BC and Frankenberger WT. Chromate reduction by chromium- resistant bacteria isolated from soil contaminated with dichromate. J. Environ. Qual. 2003;32: 1228-1233.

  • Naik UC, Srivastava S and Thakur IS. Isolation and characterization of Bacillus cereus ist105 from electroplating effluent for detoxification of hexavalent chromium. Environ. Sci. Pollut. Res. 2012; 19; 3005-3014.

  • Garbisu C, Alkorta I, Llama MJ and Serra JL. Aerobic chromate reduction by Bacillus subtilis. Biodegradation.1998; 9:133-41.

  • Thatoi H, Das S, Mishra J, Rath BP and Das N. Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review. J. Environ. Manage. 2014;146:383-399.

  • Singh KL, Kumar A and Kumar A. Short communication: Bacillus cereus capable of degrading SDS shows growth with a variety of detergents. J. Microbiol. and Biotechnol.1998;14:777-779.

  • Joshi GK, Kumar S and Sharma V. production of moderately halotolerant, SDS stable alkaline protease from Bacillus cereus MTCC 6840 isolated from lake Nainital, Uttaranchal state, India. Brazilian J. Microbiol.2007;38:773-779.

  • Sidorkina O, Saparbaev M and Laval J. Effects of nitrous acid treatment on the survival and mutagenesis of Escherichia coli cells lacking base excision repair (hypoxanthine-DNA glycosylase-ALK A protein) and/or nucleotide excision repair. Mutagenesis.1997;12:23-28.

  • Kheder AK. Study of antibiotic resistance plasmids of Pseudomonas aeruginosa, (ph.D thesis) ,University of Salah-Aldin ,Erbil ,Iraq.2002.

  • Mickelsen PA, Plorde JJ, Gordon KP, Hargiss C, McClureJ, Schoenknecht FD, Tenover FC and Tompkins LS. Instability of antibiotic resistance in a strain of Staphylococcus epidermidis isolated from an outbreak of prosthetic valve endocarditis.J.Infect.Dis.1985;152:50-58.

  • Samanta A, Bera P, Khatun M, Sinha C, Pal P, Lalee A and Mandal A. An investigation on heavy metal tolerance and antibiotic resistance properties of bacterial strain Bacillus sp. isolated from municipal waste. J. Microbiol. Biotechnol. Res. 2012;2:178-189.

  • Khatun M, Bera P, Mitra D, Mandal A and Samanta A. Estimation of heavy metal tolerance and antibiotic susceptibility of Bacillus cereus isolated from municipal solid waste. Int. J. Pharma. Bio. Sci.2012;4: (B)819-829.

  • Elsherbiny GM. Curing of plasmid –mediated antibiotics resistance in multi-drug resistant Staphylococcus sp. R.J.S. S.M.2015; 04:184-192.

  • Schott JL. The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli.J.Biol.Chem.1978;253:4341-4349.

  • Bruins MR and Oehme FW. Microbial resistance to metal in the environment. J. Ecotoxicol. Environ. Safet.1999;45:198-207.

  • Sevim A and Sevim E. Plasmid mediated antibiotic and heavy metal resistance in Bacillus strains isolated from soils in Rize, Turkey. J. Natural App.Sci.2015;19,133-141.

  • El-Mansi M, Karen JA, Inchea CA, Knowles LK and Platt DJ. Isolation and curing of the Klebsiella pneumoniae large indigenous plasmid using sodium dodecyl sulphate. Res. Microbiol.2000;51:201-208.

  • Gonzalez JM, Brown J and Carlton BC. Transfer of Bacillus thuringiensis plasmid coding for δ - endotoxin among strains Bacillus thuringiensis and Bacillus cereus. Proc. Nat. Acad. Sci. USA.1982; 79: 6951-6955.

  • Battisti, L, Green BD and Thoren C B. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis, J. Bacteriolo. 1985;162 :543-550.

  • Klee SR, Brzuszkiewicz EB, Nattermann H, Brüggemann, H, Dupke S, Wollherr A, Franz T, Pauli G, Appel B, Liebl W, Couacy-Hymann E, Boesch C, Meyer F.-D, Leendertz FH, Ellerbrok H, Gottschalk G, Grunow R and Liesegang H. The Genome of a Bacillus Isolate Causing Anthrax in Chimpanzees Combines Chromosomal Properties of B. cereus with B. anthracis Virulence Plasmids. PLoS ONE. 2010;5(7): e10986.

  • Okinaka RT and Keim P. The Phylogeny of Bacillus cereus sensulato. Microbiol. Spectr.2016;4(1). doi: 10.1128.


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  • Genetic study on Bacillus cereus

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Authors

Huda W. Hadi
Mosul University, College of Sciences, Biology Department, Iraq

Abstract


In this study, gram positive bacteria were isolated from contaminated soil in the Mosul city. Gram stain and biochemical tests were performed, which proved that the isolated bacterium was Bacillus cereus. The minimum inhibitory concentrations (MIC) of sodium chromate and sodium dodecyl sulfate (SDS) were determined to be 50 mM and 30,000μg/ml, respectively. Plasmid curing of isolated bacteria was also carried out with SDS in four sub lethal concentrations (100,200,600,1000) μg/ml, this process proved that the SDS is very efficient at 90% percentage of isolates of bacteria. The existence of plasmid was proved through plasmid extraction followed by electrophoresis. nitrous acid mutagenesis also was carried out on four intervals (15,30,45, and 60 minutes), 45 minutes was better other period on its effect on strains in their mutagenicity from chromate resistant to the chromate sensitive.

Keywords


Bacillus cereus, Plasmid, Chromate, Nitrous Acid, Resistance, SDS.

References