Research Journal of Pharmacy and Technology

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Genetic study on Bacillus cereus


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1 Mosul University, College of Sciences, Biology Department, Iraq
     

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In this study, gram positive bacteria were isolated from contaminated soil in the Mosul city. Gram stain and biochemical tests were performed, which proved that the isolated bacterium was Bacillus cereus. The minimum inhibitory concentrations (MIC) of sodium chromate and sodium dodecyl sulfate (SDS) were determined to be 50 mM and 30,000μg/ml, respectively. Plasmid curing of isolated bacteria was also carried out with SDS in four sub lethal concentrations (100,200,600,1000) μg/ml, this process proved that the SDS is very efficient at 90% percentage of isolates of bacteria. The existence of plasmid was proved through plasmid extraction followed by electrophoresis. nitrous acid mutagenesis also was carried out on four intervals (15,30,45, and 60 minutes), 45 minutes was better other period on its effect on strains in their mutagenicity from chromate resistant to the chromate sensitive.

Keywords

Bacillus cereus, Plasmid, Chromate, Nitrous Acid, Resistance, SDS.
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  • Genetic study on Bacillus cereus

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Authors

Huda W. Hadi
Mosul University, College of Sciences, Biology Department, Iraq

Abstract


In this study, gram positive bacteria were isolated from contaminated soil in the Mosul city. Gram stain and biochemical tests were performed, which proved that the isolated bacterium was Bacillus cereus. The minimum inhibitory concentrations (MIC) of sodium chromate and sodium dodecyl sulfate (SDS) were determined to be 50 mM and 30,000μg/ml, respectively. Plasmid curing of isolated bacteria was also carried out with SDS in four sub lethal concentrations (100,200,600,1000) μg/ml, this process proved that the SDS is very efficient at 90% percentage of isolates of bacteria. The existence of plasmid was proved through plasmid extraction followed by electrophoresis. nitrous acid mutagenesis also was carried out on four intervals (15,30,45, and 60 minutes), 45 minutes was better other period on its effect on strains in their mutagenicity from chromate resistant to the chromate sensitive.

Keywords


Bacillus cereus, Plasmid, Chromate, Nitrous Acid, Resistance, SDS.

References