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Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity


Affiliations
1 Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
2 Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq
     

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Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low cost-effective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.

Keywords

Penicillium italicum, Silver Nanoparticles, Antibacterial, Antifungal.
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  • Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity

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Authors

Zainab K. Taha
Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
Summaya N. Howar
Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
Ghassan M. Sulaiman
Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq

Abstract


Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low cost-effective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.

Keywords


Penicillium italicum, Silver Nanoparticles, Antibacterial, Antifungal.

References