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Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity


Affiliations
1 Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
2 Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq
     

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Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low cost-effective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.

Keywords

Penicillium italicum, Silver Nanoparticles, Antibacterial, Antifungal.
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  • - Chaturvedi S., Dave P. N., Shah N. K. Applications of nano-catalyst in new era. J. Saudi Chem. Soc., 2012, 16(3), 307-325.

  • - Darroudi M., Ahmad M.B., Zak A.K., Zamiri R., Hakimi M. Fabrication and characterization of gelatin stabilized silver nanoparticles under UV-light. Int. J. Mol. Sci., 2011, 12(9), pp.6346-6356.

  • - Kilin D.S., Prezhdo O.V., Xia Y. Shape-controlled synthesis of silver nanoparticles: Ab initio study of preferential surface coordination with citric acid. Chemical Physics Letters, 2008, 458(1-3), pp.113-116.

  • - Bansal V., Ramanathan, R., Bhargava S.K. Fungus -mediated biological approach towards ''green'' synthesis of oxide nanomaterials. Aust. J. Chem., 2011, 64:297-293.

  • - Siddiqi K. S., Husen A. Fabrication of metal nanoparticles from fungi and metal salts: scope and application. Nanoscale Res. Lett., 2016, 11(98): 1-15.

  • - Longoria E. C., Velasquez S. M., Nestor A. V., Berumen E. A., Borja M. A. Production of platinum nanoparticles and nanoaggregates using Neurosporacrassa. J. Microbiol. Biotechnol., 22(7): 1000-1004.

  • - Boroum, Moghaddam A., Namvar F., Moniri M., MdTahir P., Azizi S., Mohamad R. Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medica applications. Molecules, 2015, 20(9), pp.16540-16565 l.

  • - Louw J. P., Korsten L. Pathogenicity and host susceptibility of Penicillium spp. on citrus. Plant Disease, 2015, 99(1), 21-30.

  • - Larous L., Hendel N., Abood K., Ghoul M. The growth and production of patulin mycotoxin by Penicillium expansum on apple fruits and its control by the use of proionic acid and sodium benzoate. Arab. J.PI. Prot., 2007, 25(1):123-128.

  • - Frisvad J. C., Samson R. A. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies Mycol., 2004, 49(1): 1-174.

  • - Frisvad J. C. Phsiologyical criteria and mycotoxin production as aids in identification of common asymmetric penicillia. Appl. Environ Microbio., 1981, 41 :568-579.

  • - Pitt J.I. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces, 1979.

  • - Sadowski Z. Biosynthesis and application of silver and gold nanoparticles. In Silver Nanoparticles. In Tech, 2010.

  • - Gudikandula K., Vadapally P., Charya M. S. Biogenic synthesis of silver nanoparticles from white rot fungi: Their characterization and antibacterial studies. Open Nano, 2017, 2: 64-78.

  • - Pitt J.I. Alaboratory guide to common Penicillium species. CSIRO Division of food Processing, North Ryde, Australia, 1988.

  • - Pianzolla M. J., Moscatelli M., Vero S. Characterization of Penicillium isolates associated with blue mold on apple in Uruguay. Plant Disease, 2004, 88:23-28.

  • - Akhtar N., Anjum T., Jabeen R. Isolation and identification of storage fungi from citrus sampled from major growing areas of Punjab, Pakistan. Int. J. Agr. Biol., 2013, 15(6).

  • - Embaby E. M., Hazaa M., Hagag L. F., Ibrahim T. E., El-Azem F. A. Decay of some citrus fruit quality caused by fungi. Journal of Applied Sciences Research, 2013, 9(11): 5920-5929.

  • - Netala, V.R., Bobbu, P.L., Ghosh, S.B. and Tartte, V. Endophytic fungal assisted synthesis of silver nanoparticles, characterization, and antimicrobial activity. Asian J. Pharm. Clin. Res., 2015, 8(3): 113-116.

  • - Ali F. T., El-Sheikh H. H., El-Hady M. M., Elaasser M. M., El-Agamy, D. M. Silver Nanoparticles Synthesized by Penicilliumc itreonigrum and Fusarium moniliforme Isolated from El-Sharkia, Egypt, 2014, 5, 181-192.

  • - García M.A. Surface plasmons in metallic nanoparticles: fundamentals and applications. J. Physics D: Appl. Physics, 2011, 44(28): 283001.

  • - Abd El-Aziz E. A., Abeer R., Al-Othman M. R., Eifan S. A., Mahmoud M. A., Majrashi M. Green Synthesis of Silver Nanoparticles Using Aspergillus Terreus (KC462061). Digest J. Nanomaterials Biostruct. (DJNB), 2013, 8(3).

  • - Abdel Rahim K., Mahmoud S. Y., Ali A. M., Almaary K. S., Mustafa A. E. Z. M., Husseiny S. M. Extracellular biosynthesis of silver nanoparticles using Rhizopusstolonifer. Saudi J. Biolog. Sci., 2017, 24(1): 208-216.

  • - Villamizar-Gallardo R., Cruz J.F.O., Ortíz-Rodriguez O.O. Fungicidal effect of silver nanoparticles on toxigenic fungi in cocoa, Pesq. agropec. bras., Brasília, 2016, 51(12): 1929-1936.

  • - Li G., He D., Qian Y., Guan B., Gao S., Cui Y., Wang L. Fungus-mediated green synthesis of silver nanoparticles using Aspergillus terreus. Int. J.Mol. Sci., 2011, 13(1): 466-476.

  • - Gopinath V., Mubarak Ali D., Priyadarshini S., Priyadharsshini N. M., Thajuddin N., Velusamy P. Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids and Surfaces B: Biointerfaces, 2012, 96, 69-74.

  • - Xue B., He D., Gao S., Wang D., Yokoyama K., Wang L. Biosynthesis of silver nanoparticles by the fungus Arthrodermafulvum and its antifungal activity against Genera of Candida, Aspergillus and Fusarium. International journal of nanomedicine, 2016, 11: 1899.

  • - Singh T., Jyoti K., Patnaik A., Singh A., Chauhan R., Chandel S. S. Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus. J. Gen. Engin. Biotechnol., 2017, 15(1): 31-39.


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  • Isolation and Identification of Penicillium italicum from Iraqi Citrus Lemon Fruits and its Ability Manufacture of Silver Nanoparticles and their Antibacterial and Antifungal activity

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Authors

Zainab K. Taha
Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
Summaya N. Howar
Biology Department, College of Education for Pure Sciences/Ibn al-Haitham, University of Baghdad, Baghdad, Iraq
Ghassan M. Sulaiman
Biotechnology Division, Applied Science Department, University of Technology, Baghdad, Iraq

Abstract


Recently, attention to the manufacture of nanoparticles has been vital as a result to develop safe, low cost-effective and environmentally friendly methods for nanoparticle manufacturing techniques. In this study, Penicillium italicum was isolated and diagnosed from Iraqi citrus lemon fruits using various media including PDAs, MEA, CDA, Nitrite sucrose (no2) Agar, CYA and YES. Isolates were cultured at different temperatures of 5, 25 and 37°C to diagnose the morphological characteristics of P. italicum, and then using P. italicum in the production of silver nanoparticles using X-Ray diffraction and Atomic Force Microscope. The antifungal and antibacterial activity was determined against Cladosporium sp, Monilia sp., Fusarium sp., Candida glabrata and Klebsiella pneumonia. The results have been shown an inhibition effect of silver nanoparticles produced by P. italicumon the growth of studies bacteria and fungi in dose-dependent manner.

Keywords


Penicillium italicum, Silver Nanoparticles, Antibacterial, Antifungal.

References