Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Inhibitory Potential of Amaranthus viridis on Α-Amylase and Glucose Entrapment Efficacy In vitro


Affiliations
1 Department of Biotechnology, Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
2 Department of Biotechnology, Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India. (Research centre-Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
     

   Subscribe/Renew Journal


Type 2 Diabetes which prevails globally increasing at an alarming rate, is characterized by hyperglycemia where blood glucose levels remain elevated due to poor cellular glucose uptake and metabolism. One way to treat this disease is by α-amylase inhibitors and glucose retention in intestine for longer period. The present study was designed to determine the in vitro antidiabetic potential of Amaranthus virdis extracts. Antidiabetic action was confirmed by the inhibitory effects of the A.viridis extracts on α-amylase activity and significant glucose entrapment ability. The methanolic extract of A. viridis leaves exhibited the highest glucose retention ability of 47.05% (40.125μg/mL) within the dialysis tube which mimics human intestine. Water extract of A. Viridis stem showed the most significant inhibition effect on α-amylase activity (100%) compared to other extracts after 15 minutes of incubation, with IC50 value of (5.058±0.41μg/mL) and which was even equally active to the standard acarbose drug used in the current analysis. Interestingly, the methanol extract of leaf exhibited notable efficacy both on glucose diffusion inhibition and inhibition of alpha amylase as well. This study suggests for further in vivo research to check their activity and toxicity within biological systems and also for the isolation of bioactive inhibitory compounds for novel drug discovery.

Keywords

Antidiabetic Activity, Amaranthus viridis, In vitro, Glucose Diffusion Inhibition, Alpha Amylase Inhibition.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Rines AK, Sharabi K, Tavares CDJ and Puigserver. Targeting hepatic glucose metabolism in the treatment of type 2 diabetes. Nature Reviews Drug Discovery, 15; 2016: 786–804.

  • Somwanshi SB, Bairagi PD and Kotade KB. Asian Study of Gestational Diabetes Mellitus: A Brief Review Journal of Pharmacy Research,7(2); 2017: 118-123.

  • Sue Kirkman M, Vanessa Jones Briscoe, Nathaniel Clark, Hermes Florez, Linda B. Haas, Jeffrey B. Halter, Elbert S. Huang, Mary T. Korytkowski, Medha N. Munshi, Peggy Soule Odegard, Richard E. Pratley and Carrie S. Swift. Diabetes in older adults. Diabetes Care. 35; 2012: 2650–2664.

  • Khan MY, Irfan Aziz, Bipin Bihari, Hemant Kumar, Maryada Roy and Vikas Kumar Verma. A Review- Phytomedicines Used in Treatment of Diabetes.Asian Journal of Pharmaceutical Research, 4(3);2014: 135-154.

  • International Diabetes Federation. IDF Diabetes ATLAS. In: International Diabetes Federation. Available from http://www.diabetesatlas.org/. Accessed on 29thSeptember 2017.

  • Roshani Bhalerao, Akshay Patil, Dinesh Rishipathak and Sanjay Kshirsagar.Insulin Therapies: Current and Future Trends.Asian Journal of Research in Pharmaceutical Sciences,7(4); 2017: 189-196.

  • Amy Bradshaw Kaiser, Nicole Zhang and Wouter Van Der Pluijm. Global Prevalence of Type 2 Diabetes over the Next Ten Years (2018-2028). Diabetes. 67 (1); 2018: 202.

  • Harikrishnan V, Sreejith M, Elthose MJ and Akash Marathakam. Gelucire: An Optional Innovative Tool for both Sustained and Fast Release of Drugs in Treating Diabetes Mellitus Type II Disease.Asian Journal of Research in Pharmaceutical Sciences, 7(1); 2017: 33-37.

  • Ali H, Houghton P, and Soumyanath A. Alpha amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology.107(3); 2006:449-455.

  • Pattabiraman K and Muthukumaran P. Antidiabetic and Antioxidant Activity of Morinda tinctoria roxb Fruits Extract in Streptozotocin-Induced Diabetic Rats.Asian Journal of Pharmacy and Technology, 1(2); 2011: 34-39.

  • Edwards CA, Black burn NA, Craigne L, Daavidson P, Tomlin J, Sugden K, Johnson IT and Read NW. Viscosity of food gums determined in vitro related to their hypoglycemic actions. The American Journal of Clinical Nutrition.46; 1987: 72-77.

  • Tanushree Chatterjee, Pradeep Kumar Sahu, Shilpi Chatterjee and Jai Godheja. Current and Future Biotechnological Approaches for Diabetes Mellitus.Asian Journal of Pharmacy and Technology, 1(4); 2011: 94-98.

  • Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. The potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary Alternative Medicines.11; 2011: 5.

  • Jaya Preethi P. Herbal Medicine for Diabetes Mellitus: A Review. Asian Journal of Pharmaceutical Research, 3(2); 2013: 57-70.

  • Duncan DB. Multiple range and multiple F test. Biometrics.11; 1955: 1–42.

  • Khan MY, Poonam Gupta, Bipin Bihari, Vikas Kumar and Verma. A Review on Diabetes and Its Management.Asian Journal of Pharmaceutical Research, 3(1); 2013; 28-33.

  • Qujeq D, and Babazadeh A. The entrapment ability of aqueous and ethanolic extract of Teucrium polium: glucose diffusion into the external solution. International Journal of Molecular and Cellular Medicine. 2(2); 2013: 93.

  • Dharmesh Sharm, Deepak Prashar and Sanjay Saklani.Bird’s Eye View on Herbal Treatment of Diabetes. Asian Journal of Pharmaceutical Research, 2(1);2012: 01-06.

  • Archit R, Gayathri M, and Punnagai M. An in vitro investigation into the mechanism of anti-diabetic activity of selected medicinal plants. International Journal of Drug Development and Research.9 (2); 2013: 2.

  • He L. Alpha-glucosidase inhibitors as agents in the treatment of diabetes. Diabetes Review, 6; 1998: 132-145.

  • Sundarrajan T, Velmurugan V and Srimathi R. Phytochemical Evaluation and In Vitro Antidiabetic Activity of Ethanolic extract of Alternanthera ficodia Linn. Research Journal of Pharmacy and Technology, 10 (9); 2017: 2981-2983.

  • Vivek Kumar R, Satish kumar, Shashidhara S and Anitha S. In-Vitro Anti-Oxidant, Anti-Amylase, Anti-Arthritic and Cytotoxic Activity of Important Commonly Used Green Leafy Vegetables. International Journal of PharmTech Research. 3 (4); 2011: 2096-2103.


Abstract Views: 260

PDF Views: 0




  • Inhibitory Potential of Amaranthus viridis on Α-Amylase and Glucose Entrapment Efficacy In vitro

Abstract Views: 260  |  PDF Views: 0

Authors

P. A. Mary Helen
Department of Biotechnology, Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
B. Jesvin Bency
Department of Biotechnology, Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India. (Research centre-Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India

Abstract


Type 2 Diabetes which prevails globally increasing at an alarming rate, is characterized by hyperglycemia where blood glucose levels remain elevated due to poor cellular glucose uptake and metabolism. One way to treat this disease is by α-amylase inhibitors and glucose retention in intestine for longer period. The present study was designed to determine the in vitro antidiabetic potential of Amaranthus virdis extracts. Antidiabetic action was confirmed by the inhibitory effects of the A.viridis extracts on α-amylase activity and significant glucose entrapment ability. The methanolic extract of A. viridis leaves exhibited the highest glucose retention ability of 47.05% (40.125μg/mL) within the dialysis tube which mimics human intestine. Water extract of A. Viridis stem showed the most significant inhibition effect on α-amylase activity (100%) compared to other extracts after 15 minutes of incubation, with IC50 value of (5.058±0.41μg/mL) and which was even equally active to the standard acarbose drug used in the current analysis. Interestingly, the methanol extract of leaf exhibited notable efficacy both on glucose diffusion inhibition and inhibition of alpha amylase as well. This study suggests for further in vivo research to check their activity and toxicity within biological systems and also for the isolation of bioactive inhibitory compounds for novel drug discovery.

Keywords


Antidiabetic Activity, Amaranthus viridis, In vitro, Glucose Diffusion Inhibition, Alpha Amylase Inhibition.

References