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Inhibitory Potential of Amaranthus viridis on Α-Amylase and Glucose Entrapment Efficacy In vitro


Affiliations
1 Department of Biotechnology, Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
2 Department of Biotechnology, Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India. (Research centre-Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
     

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Type 2 Diabetes which prevails globally increasing at an alarming rate, is characterized by hyperglycemia where blood glucose levels remain elevated due to poor cellular glucose uptake and metabolism. One way to treat this disease is by α-amylase inhibitors and glucose retention in intestine for longer period. The present study was designed to determine the in vitro antidiabetic potential of Amaranthus virdis extracts. Antidiabetic action was confirmed by the inhibitory effects of the A.viridis extracts on α-amylase activity and significant glucose entrapment ability. The methanolic extract of A. viridis leaves exhibited the highest glucose retention ability of 47.05% (40.125μg/mL) within the dialysis tube which mimics human intestine. Water extract of A. Viridis stem showed the most significant inhibition effect on α-amylase activity (100%) compared to other extracts after 15 minutes of incubation, with IC50 value of (5.058±0.41μg/mL) and which was even equally active to the standard acarbose drug used in the current analysis. Interestingly, the methanol extract of leaf exhibited notable efficacy both on glucose diffusion inhibition and inhibition of alpha amylase as well. This study suggests for further in vivo research to check their activity and toxicity within biological systems and also for the isolation of bioactive inhibitory compounds for novel drug discovery.

Keywords

Antidiabetic Activity, Amaranthus viridis, In vitro, Glucose Diffusion Inhibition, Alpha Amylase Inhibition.
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  • Inhibitory Potential of Amaranthus viridis on Α-Amylase and Glucose Entrapment Efficacy In vitro

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Authors

P. A. Mary Helen
Department of Biotechnology, Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India
B. Jesvin Bency
Department of Biotechnology, Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India. (Research centre-Malankara Catholic College, Kanyakumari District-629153, Tamilnadu, India

Abstract


Type 2 Diabetes which prevails globally increasing at an alarming rate, is characterized by hyperglycemia where blood glucose levels remain elevated due to poor cellular glucose uptake and metabolism. One way to treat this disease is by α-amylase inhibitors and glucose retention in intestine for longer period. The present study was designed to determine the in vitro antidiabetic potential of Amaranthus virdis extracts. Antidiabetic action was confirmed by the inhibitory effects of the A.viridis extracts on α-amylase activity and significant glucose entrapment ability. The methanolic extract of A. viridis leaves exhibited the highest glucose retention ability of 47.05% (40.125μg/mL) within the dialysis tube which mimics human intestine. Water extract of A. Viridis stem showed the most significant inhibition effect on α-amylase activity (100%) compared to other extracts after 15 minutes of incubation, with IC50 value of (5.058±0.41μg/mL) and which was even equally active to the standard acarbose drug used in the current analysis. Interestingly, the methanol extract of leaf exhibited notable efficacy both on glucose diffusion inhibition and inhibition of alpha amylase as well. This study suggests for further in vivo research to check their activity and toxicity within biological systems and also for the isolation of bioactive inhibitory compounds for novel drug discovery.

Keywords


Antidiabetic Activity, Amaranthus viridis, In vitro, Glucose Diffusion Inhibition, Alpha Amylase Inhibition.

References