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Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation


Affiliations
1 Department of Laboratory Investigation, Kufa University, Najaf, Iraq
     

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Human Papillomavirus was is olated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papillomavirus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papilloma virus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo [specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50 /0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords

HPV, RT-PCR, TCID50, SPF.
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  • Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation

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Authors

Fadyia Mahdi Muslim Alameedy
Department of Laboratory Investigation, Kufa University, Najaf, Iraq

Abstract


Human Papillomavirus was is olated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papillomavirus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papilloma virus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo [specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50 /0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords


HPV, RT-PCR, TCID50, SPF.

References