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Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation


Affiliations
1 Department of Laboratory Investigation, Kufa University, Najaf, Iraq
     

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Human Papillomavirus was is olated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papillomavirus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papilloma virus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo [specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50 /0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords

HPV, RT-PCR, TCID50, SPF.
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  • Sankaranarayanan R, Swaminathan R, Brenner H, Chen K, Chia KS, Chen JG, et al. Cancer survival in Africa, Asia, and Central America: a population-based study. Lancet Oncology. 2010, 11:165–173.

  • Kraut AA, Schink T, Schulze-Rath R, Mikolajczyk RT, Garbe E. Incidence of anogenital warts in Germany: a population-based cohort study. BMC Infect Dis. 2010, 10:360.

  • Hemminki K, Li X, Vaittinen P. Time trends in the incidence of cervical and other genital squamous cell carcinomas and adenocarcinomas in Sweden, 1958-1996. Eur J Obstet Gynecol Reprod Biol. 2002, 101:64–69.

  • Wilkinson JR, Morris EJ, Downing A, Finan PJ, Aravani A, Thomas JD, et al. The rising incidence of anal cancer in England 1990-2010: a population-based study. Colorectal dis. 2014, 16: O234–O239.

  • Petersen I, Klein F. HPV in non-gynecological tumors. Pathologe. 2008, 29:118–122. German.

  • Erol D, Bulut Y, Yuce H, Ozercan IH. Investigation of the presence of human papillomavirus DNA in various gastrointestinal carcinoma samples. Mikrobiyol Bul. 2009, 43:259–268. Turkish.

  • Mlynarczyk B, Malejczyk M, Muszynski J, Majewski S. The occurrence of human papillomavirus--HPV in the biopsies from colon polyps and cancer. Med Dosw Mikrobiol. 2009, 61:191–196. Polish.

  • General Information About Colon Cancer. NCI. May 12, 2014. Archived from the original on July 4, 2014. Retrieved June 29, 2014.

  • World Cancer Report 2014. World Health Organization. 2014. pp. Chapter 5.5. ISBN 9283204298.

  • Colorectal Cancer Prevention. National Cancer Institute. February 27, 2014. Archived from the original on July 5, 2014. Retrieved June 29, 2014.

  • Colon Cancer Treatment. NCI. May 12, 2014. Archived from the original on July 5, 2014. Retrieved June 29, 2014.

  • OIE." Avian influenza. Chapter 2.3.4". OIE (world Organization for animal health

  • Terrestrial Manual. Pp:43-56; 78-89; 143-146. (2009). Moresco, K. A., Stallknecht, D. E. and Swayne, D. E. "Evaluation and Attempted Optimization of Avian Embryos and Cell Culture Methods for Efficient Isolation and Propagation of Low Pathogenicity Avian Influenza Viruses" Avian Diseases Digest, vol. 5, no. s1, p. e142 -e143(2010).

  • Frey, H.R. and Liess, B." Growth Curve Studies and Applicability of Highly Cytopathogenic BVD-MD Virus Strain for Diagnosis Purposes Using Microtiter Method". ZBL. Vet. Med. 18:16-71. (1971).

  • Knoll, G. F. (1979). Radiation Detection and Measurement (John Wiley& Sons, New York, Pp35-37.

  • Kleter, B. Quint, W. Molijn, A and Doorn,L. Molecular diagnosis of human papillomavirus (HPV) infections Journal of Clinical Virology 32S (2005) S43–S51

  • Sohrab, B., Koji ,Y. Shu,Y. Maria, D. Joel ,M. and Zhi-Ming Z. Colorectal papillomavirus infection in colorectal cancer patients. Clin Cancer Res. 2005 Apr 15; 11(8): 2862–2867.

  • Andrea, N. Polly, A. Margaret, T. Denise, A. Margaret, M. Michelle, A. Joseph, J. Karen, W. John, D. and Stephen, M. No evidence for human papillomavirus in the etiology of colorectal polyps. Cancer Epidemiol Biomarkers Prev. 2011 Oct; 20(10): 2288–2297.

  • Brenner H, Hoffmeister M, Stegmaier C, Brenner G, Altenhofen L, Haug U. Risk of progression of advanced adenomas to colorectal cancer by age and sex: estimates based on 840,149 screening colonoscopies. Gut. 2007;56(11):1585–1589.

  • Damin, D. Ziegelmann, P and Damin, A. Human papilloma virus infection and colorectal cancer risk: a meta-analysis. Colorectal Disease Volume 15, Issue 8, Version of Record online: 29 AUG 2013.

  • Gresser, I. Chany, C and Enders, J. Persistent Polioviral Infection of Intact Human Amniotic Membrane Without Apparent Cytopathic Effect. Journal of Bacteriology, Feb., 1965. American Society for Microbiology Vol. 89, No. 2 Printed in U.S.A.


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  • Diagnosis of Human Papillomavirus in Colorectal Cancer and Inhibition by Gamma Radiation

Abstract Views: 235  |  PDF Views: 0

Authors

Fadyia Mahdi Muslim Alameedy
Department of Laboratory Investigation, Kufa University, Najaf, Iraq

Abstract


Human Papillomavirus was is olated from different age groups from 40 upto 71 years for both gender. The samples were diagnosed by RT-PCR. Positive cases of human Papillomavirus were forty represent (63.49%) and twenty two represent (36.50%) negative cases. Isolation process of Human Papilloma virus was successfully performed on chicken embryo fibroblast cell culture for 4th passage for suspected samples obtained from (colon organ) as well as isolate from chick embryo [specific pathogen free (SPF). It was found that at the tissue culture infective dosage (TCID50 /0.1 ml) was (107.8) of virus. The gamma irradiation that dosage used for inactivating of the purified virus which was extracted from allantoic fluid and tissue culture was (6.594) mSv/h. This revision intended to gauge the prevalence of HPV cannon and viral incorporation in colorectal tissues in imperative to estimate its presumed role in colorectal cancer and inhibition viral by gamma radiation.

Keywords


HPV, RT-PCR, TCID50, SPF.

References