Research Journal of Pharmacy and Technology

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Parthenium-turmeric Ointment: A Novel Approach for Excision Wound Healing on Rabbits


Affiliations
1 Department of Pharmaceutical Technology, Bengal School of Technology (A College of Pharmacy), Delhi Road, Sugandha, Hoogly, Pin-712102, West Bengal,, India
2 Department of Pharmaceutics, BCDA College of Pharmacy and Technology, 78/1 Jessore Road (S), Hridaypur, Barasat, Kolkata 700127, West Bengal, India
3 Bengal College of Pharmaceutical Sciences and Research, Basu Sarani, Burdwan, Bidhannagar, Durgapur, Pin-713212, West Bengal,, India
4 Department of Pharmaceutical Technology, Brainware University, 398, Ramkrishnapur Road, Barasat, Kolkata 700125, West Bengal,, India
     

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Traditional phytomedicines owing to their affordable price and fewer side effects over synthetic preparations may have a wide range of applications in primary health care system. However, there was no scientific report documented so far on the excision wound healing activity of Parthenium hysterophorus-Turmeric ethanolic extract on rabbits. The present study deals with the formulation and evaluation of Parthenium hysterophorus-Turmeric ointment in definite ratio. The four batches were examined for hemocompatibility study and characterised for physical appearance, pH, rheological behaviour, in-vitro drug diffusion pattern, skin irritation excision wound healing and stability study. All the hemocompatible herbal formulations were found to possess satisfactory organoleptic properties and applicability parameters. An inverse relationship was observed between viscosity and % spreadability of ointments. Non-Fickian diffusion based controlled drug release was observed for all these topical preparations. Excision wound healing activity was assessed on the basis of % wound contraction, duration of epithelisation and tensile strength. PHTO1 (ethanolic extract of Parthenium hysterophorus: Turmeric-3:2) showed greater (1.10-1.34 times) wound healing activity compared to only Parthenium extract containing ointment (PHO1). Tissue debride effect was also observed after 7th day of applications of each formulation. All the prepared Parthenium-Turmeric formulations were found to be stable suggesting elaborative research to confirm the pharmacologically active constituent responsible for excision wound healing activity.

Keywords

Epithelisation, Excision wound healing, Non-Fickian diffusion, Parthenium hysterophorus, Tissue debride effect, Wound contraction
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  • Swamy HK, Krishna V, Shankarmurthy K et al. Wound healing activity of embelin isolated from the ethanol extract of leaves of Embelia ribes Burm. Journal of Ethnopharmacology. 2007; 109(3): 529-34.

  • Harish B, Krishna V, Kumar HS et al. Wound healing activity and docking of glycogen-synthase-kinase-3-β-protein with isolated triterpenoid lupeol in rats. Phytomedicine. 2008; 15(9): 763-7.

  • Sharath R, Harish B, Krishna V et al. Wound healing and protease inhibition activity of Bacoside‐A, isolated from Bacopa monnieri wettest. Phytotherapy Research. 2010; 24(8): 1217-22.

  • Lingaraju G, Krishna V, JoyHoskeri H et al. Wound healing promoting activity of stem bark extract of Semecarpus anacardium using rats. Natural Product Research. 2012; 26(24): 2344-2347.

  • Ingole NA, Thaware PP, Verma SK et al. Study of wound healing activity of Parthenium hysterophorus by excision wound model in rats. International Journal of Medical Research Professionals. 2018; 4(2); 308-13.

  • Kumar PK, Govindasamy K, Kumaresan G et al. A critical review on traditional medicines, ayurvedic herbs and fruits in the treatment of cardiovascular disease. Research Journal of Pharmacy and Technology. 2020; 13(7): 3480-4.

  • Pavithra HD, Vishnupriya V, Gayathri R. Herbal remedies for anxiety and depression-a review. Research Journal of Pharmacy and Technology. 2016; 9(8): 1253-6.

  • Aswathi T, Venkateswaramurthy N, Sambath KR. A review on relevance of herbal medications for psychiatric patients. Research Journal of Pharmacy and Technology. 2019; 12(7): 3151-6.

  • Bura AR. Effect of woun healing potential of Plumeria obtuse (Champa) spray: Asian Journal of Pharmaceutical Research. 2018; 8(4): 231-5.

  • Purohit SK, Solanki R, Soni R et al. Evaluation of wound healing activity of ethanolic extract of Azadirachta indica leaves in male albino rats: Asian Journal of Pharmacy and Technology. 2013; 3(2): 73-5.

  • Shetty P, Chacko N, Alva A et al. Wound heaing potential of Psidium guajava var. pyrifera: Research Journal of Pharmacy and Technology. 2019; 12(12): 6067-70.

  • Krishnaveni M, Kalaivani M, Ragina Banu C et al. GC-MS/MS study of Parthenium hysterophorus L (N.Am) stem, antimicrobial activity: Research Journal of Pharmacy and Technology. 2015; 8(5): 517-9.

  • Mahato TK, Sharma K. Study of medicinal herbs and its antibacterial activity: A review. Journal of Drug Delivery and Therapeutics. 2018; 8(5S): 47-54.

  • Bagchi A, Raha A, Mukherjee P. A complete review on Parthenium hysterophorus Linn. International Journal of Recent Advances in Pharmaceutical Research. 2016; 6(1): 42-9.

  • Dutta A, Ghosh B. In-vitro antimicrobial activity of Parthenium hysterophorus comparison with ofloxacin. International Journal of Pharmaceutical Sciences Review and Research. 2020; 62(1): 49-55.

  • Kumar S, Mishra A, Pandey AK. Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complementary and Alternative Medicine. 2013; 13: 9pgs.

  • Wondmu A, Bassazinew S, Muktar Y. In-vitro anthelmintic activities of crude extraction of Parthenium hysterophorus against Haemonchus contortus. Journal of Biology, Agriculture and Healthcare. 2017; 7(7): 72-7.

  • Saleem M, Khan A, Naureen H et al. Protective effect of Parthenium hysterophorus against carbon tetrachloride- and paracetamol-induced hepatotoxicity in rabbits. Tropical Journal of Pharmaceutical Research. 2018; 17(3): 467-73.

  • Kumari C, Deepalakshmi J. Phytoextraction of lead from soil by Parthenium hysterophorus Linn: Research Journal of Pharmacy and Technology. 2017; 9(2): 64-8.

  • Kumari C, Deepalakshmi J. Qualitative and GC-MS analysis of phytoconstituents of Parthenium hysterophorus Linn: Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(2): 105-10.

  • Krishnaveni M, Kalaivani M, Krishnakumari G. Phytoanalysis of Parthenium hysterophorus L root, stem: Research Journal of Pharmacy and Technology. 2015; 8(12): 1685-90.

  • Marimuthu K, Kalaivani M, Krishnakumari G. Phytoanalysis of Parthenium hysterophorous L root, stem. Research Journal of Pharmacy and Technology.2015; 8(12): 1685-90.

  • Hewlings SJ, Kalman DS. Curcumin: A review of it’s effects on human health. Foods. 2017; 6: 11pgs.

  • Tanvir EM, Hossen MS, Hossain MF et al. Antioxidant properties of popular turmeric (Curcuma longa) varieties from Bangladesh. Journal of Food Quality. 2017; 3: 8pgs.

  • Jayandran M, Muhamed Haneefa M, Balasubramanian V. Synthesis, characterization and antimicrobial activities of turmeric curcumin and curcumin stabilized zinc nanoparticles-a green approach. Research Journal of Pharmacy and Technology. 2015; 8(4): 445-51.

  • Vinitha S, Geetha RV. In vitro anti inflammatory activity of methanolic extract of Curcuma longa. Research Journal of Pharmacy and Technology. 2015; 8(7): 943-4.

  • Pattanayak S, Nayak SS, Dinda SC et al. Evaluation of herbal ointments formulated with methanolic extract of Cajanus scarabaeoides. Journal of Pharmacy and Allied Health Sciences. 2011; 1(2): 49-57.

  • Ravishankar K, Kiranmayi GVN, Prasad YR et al. Wound healing activity in rabbits and antimicrobial activity of Hibiscus hirtus ethanolic extract. Brazilian Journal of Pharmaceutical Sciences. 2018; 54(4): 12 pgs.

  • Deshmukh R, Agarwal R, Chauragde S et al. Formulation and evaluation of ointment containing natural wound healing activity of Tridax procumbens. Research Journal of Pharmacy and Technology. 2018; 11(10): 4543-6.

  • Mukherjee S, Ash D, Majee SB et al. Studies on Span based soy-bigels with HPMC. Research Journal of Pharmacy and Technology. 2020; 13(1): 353-60.

  • Bartosova L, Bajgar J. Transdermal drug delivery in vitro diffusion cells. Current Medicinal Chemistry. 2012; 19: 4671- 7.

  • Roy H, Balaiah S, VinayKumar T. Design and in-vitro evaluation of cefuroxime axetil floating microbeads for treatment of acute bacterial caused chronic bronchitis: Research Journal of Pharmacy and Technology. 2018; 11(6): 2276-82.

  • Patil SS, Bhasarkar S, Rathod VK. Extraction of curcuminoids from Curcuma longa: comparative study between batch extraction and novel three phase partitioning. Preparative Biochemistry and Biotechnology. 2019; 49(4): 407-18.

  • Purohit SK, Solanki R, Mathur V et al. Evaluation of wound healing activity of ethanolic extract of Curcuma longa rhizomes in male albino rats. Asian Journal of Pharmaceutical Research. 2013; 3(2): 79-81.

  • Awad El-Gied AA, Abdelkareem AM, Hamedelniel EI. Investigation of cream and ointment on antimicrobial activity of Mangifera indica extract. Journal of Advanced Pharmaceutical Technology and Research. 2015; 6(2): 53-7.

  • Iba Y, Shibata A, Kato M et al. Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice. International Immunopharmacology. 2004; 4(14): 1873-80.

  • Stadelmann WK, Digenis AG, Tobin GR. Impediments to wound healing. The American journal of surgery. 1998; 176(2): 39S-47S.

  • Surale-Patil SA, Patil TR. Comparative study of wound healing potential of glyceryl trinitrate, diltiazem and rapan tail in wistar rats: Research Journal of Pharmacy and Technology. 2020; 13(9): 4395-8.

  • Rao C, Ghosh A. Does metronidazole reduce lipid peroxidation in burn injuries?. Indian Journal of Pharmacology. 1997; 29: 30-2.


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  • Parthenium-turmeric Ointment: A Novel Approach for Excision Wound Healing on Rabbits

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Authors

Ayon Dutta
Department of Pharmaceutical Technology, Bengal School of Technology (A College of Pharmacy), Delhi Road, Sugandha, Hoogly, Pin-712102, West Bengal,, India
Dipanjana Ash
Department of Pharmaceutics, BCDA College of Pharmacy and Technology, 78/1 Jessore Road (S), Hridaypur, Barasat, Kolkata 700127, West Bengal, India
Animesh Roy
Bengal College of Pharmaceutical Sciences and Research, Basu Sarani, Burdwan, Bidhannagar, Durgapur, Pin-713212, West Bengal,, India
Piyali Khamkat
Department of Pharmaceutical Technology, Brainware University, 398, Ramkrishnapur Road, Barasat, Kolkata 700125, West Bengal,, India
Abhishek Ghosh
Bengal College of Pharmaceutical Sciences and Research, Basu Sarani, Burdwan, Bidhannagar, Durgapur, Pin-713212, West Bengal,, India

Abstract


Traditional phytomedicines owing to their affordable price and fewer side effects over synthetic preparations may have a wide range of applications in primary health care system. However, there was no scientific report documented so far on the excision wound healing activity of Parthenium hysterophorus-Turmeric ethanolic extract on rabbits. The present study deals with the formulation and evaluation of Parthenium hysterophorus-Turmeric ointment in definite ratio. The four batches were examined for hemocompatibility study and characterised for physical appearance, pH, rheological behaviour, in-vitro drug diffusion pattern, skin irritation excision wound healing and stability study. All the hemocompatible herbal formulations were found to possess satisfactory organoleptic properties and applicability parameters. An inverse relationship was observed between viscosity and % spreadability of ointments. Non-Fickian diffusion based controlled drug release was observed for all these topical preparations. Excision wound healing activity was assessed on the basis of % wound contraction, duration of epithelisation and tensile strength. PHTO1 (ethanolic extract of Parthenium hysterophorus: Turmeric-3:2) showed greater (1.10-1.34 times) wound healing activity compared to only Parthenium extract containing ointment (PHO1). Tissue debride effect was also observed after 7th day of applications of each formulation. All the prepared Parthenium-Turmeric formulations were found to be stable suggesting elaborative research to confirm the pharmacologically active constituent responsible for excision wound healing activity.

Keywords


Epithelisation, Excision wound healing, Non-Fickian diffusion, Parthenium hysterophorus, Tissue debride effect, Wound contraction

References