Research Journal of Pharmacy and Technology

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Anti-inflammatory Evaluation of Moringa-albumin Combination in Inhibiting Ifn-γ and Tnf-α Expression in Diabetic Mouse Model


Affiliations
1 Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
2 Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550,, Japan
     

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This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.

Keywords

Albumin, Diabetes Mellitus, IFN-γ. Moringa oleifera, TNF-α,.
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  • International Diabetes Federation. IDF Diabetes Atlas. Brussels: International Diabetes Federation. 2011; 5th ed.

  • International Diabetes Federation. IDF Diabetes Atlas. Brussels: International Diabetes Federation. 2013; 6th ed.

  • International Diabetes Federation. IDF Diabetes Atlas. Brussels: International Diabetes Federation. 2015; 7th ed.

  • Cho NH, Shaw JE, Karuranga S, Huang Y, da Rocha Fernandes JD, Ohlrogge AW, Malanda B. IDF Diabetes Atlas: Global Estimates of Diabetes Prevalence for 2017 and Projections for 2045. Diabetes Research and Clinical Practice. 2018;138: 271-281.

  • Baena-Díez JM, Peñafiel J, Subirana I, Ramos R, Elosua R, Marín-Ibañez A, et al. Risk of Cause-Specific Death in Individuals with Diabetes: a Competing Risks Analysis. Diabetes Care. 2016; 39(11): 1987-1995.

  • King GL. The Role of Inflammatory Cytokines in Diabetes and Its Complications. Journal of Periodontology. 2008; 79(8s): 1527-1534.

  • Cruz NG, Sousa LP, Sousa MO, Pietrani NT, Fernandes AP, Gomes KB. The Linkage between Inflammation and Type 2 Diabetes Mellitus. Diabetes Research and Clinical Practice. 2013; 99(2): 85-92.

  • Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. Adipose Tissue Tumor Necrosis Factor and Interleukin-6 Expression in Human Obesity and Insulin Resistance. American Journal of Physiology-Endocrinology and Metabolism. 2001; 280(5): 745-751.

  • Antony J, Debroy S, Manisha C, Thomas P, Jeyarani V, Choephel T. In-vitro Cell Line Models and Assay Methods to Study the Anti-diabetic Activity. Research Journal of Pharmacy and Technology. 2019; 12(5): 2200-2206.

  • Kashiwagi A. Complications of Diabetes Mellitus and Oxidative Stress. Japan Medical Association Journal. 2001; 44(12): 521-528.

  • Giacco F, Brownlee M. Oxidative Stress and Diabetic Complications. Circulation Research. 2010; 107(9): 1058-1070.

  • de M Bandeira S, da Fonseca LJ, da S Guedes G, Rabelo LA, Goulart MO, Vasconcelos SM. Oxidative Stress as an Underlying Contributor to the Development of Chronic Complications in Diabetes Mellitus. International Journal of Molecular Sciences. 2013; 14(2): 3265-3284.

  • Manguro LO, Lemmen P. Phenolics of Moringa oleifera Leaves. Natural Product Research. 2007; 21(1): 56-68.

  • Kashiwada Y, Ahmed FA, Kurimoto S, Kim SY, Shibata H, Fujioka T, Takaishi Y. New α-Glucosides of Caffeoylquinic Acid from the Leaves of Moringa oleifera Lam. Journal of Natural Medicines. 2012; 66(1): 217-221.

  • Dhimmar N, Patel NM, Gajera V, Lambole V. Pharmacological Activities of Moringa oleifera: An Overview. Research Journal of Pharmacy and Technology. 2015; 8(4): 476-480.

  • Makkar HPS, Becker K. Nutrients and Anti Quality Factors in Different Morphological Parts of the Moringa oleifera tree. The Journal of Agricultural Science. 1997; 128(3): 311-322.

  • Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A Food Plant with Multiple Medicinal Uses. Phytotherapy Research. 2007; 21(1): 17-25.

  • Farooq F, Rai M, Tiwari A, Khan AA, Farooq S. Medicinal Properties of Moringa oleifera: An Overview of a Promising Healer. Journal of Medicinal Plants Research. 2012; 6(27): 4368-4374.

  • Das AK, Rajkumar V, Verma AK, Swarup D. Moringa oleifera Leaves Extract: A Natural Antioxidant for Retarding Lipid Peroxidation in Cooked Goat Meat Patties. International Journal of Food Science and Technology. 2012. 47(3): 585-591.

  • Moyo B. Antimicrobial Activities of Moringa oleifera Lam Leaf Extracts. African Journal of Biotechnology. 2012; 11(11): 2797-2802.

  • Toma A, Deyno S. Phytochemistry and Pharmacological Activities of Moringa oleifera. International Journal of Pharmacognosy. 2014; 1(4): 222-231.

  • Dwijayanti DR, Djati MS, Rifa’i M. The Role of VipAlbumin® as an Immunostimulatory Agent for Controlling Homeostasis and Proliferation of Lymphoid Cells. Central European Journal of Immunology. 2016; 41(1): 31-38.

  • Narwadiya SC, Dhumne UL, Sahare KN, Tuman PM, Meshram VG, Singh V. Serum Protein Level Changes in Dots Administered Patients of Nagpur District: A Case Study. India Asian J Exp BiolScienst. 2012; 3: 251-254.

  • Shi H, Clegg DJ. Sex differences in the regulation of body weight. Physiology & Behavior. 2009; 97(2): 199-204.

  • Brosius F. High-dose Streptozotocin Induction Protocol (Mouse). 2015; Diabetic Complications Consortium. Available from: https://www.diacomp.org/ (Jul. 3, 2020).

  • Reusser F. Mode of Action of Streptozotocin. Journal of Bacteriology. 1971; 105(2): 580-588.

  • Singh SN, Vats P, Suri S, Shyam R, Kumria MML, Ranganathan S. Effect of an Antidiabetic Extract of Catharanthus roseus on Enzymic Activities in Streptozotocin-induced Diabetic Rats. Journal of Ethnopharmacology. 2001; 76(3); 269-277.

  • Engsuwan J, Waranuch N, Limpeanchob N, Ingkaninan K. HPLC Methods for Quality Control of Moringa oleifera Extract using Isothiocyanates and Astragalin as Bioactive Markers. ScienceAsia. 2017; 43(3): 169-174.

  • Luqman S, Srivastava S, Kumar R, Maurya AK, Chanda D. Experimental Assessment of Moringa oleifera Leaf and Fruit for Its Antistress, Antioxidant, and Scavenging Potential Using In Vitro and In Vivo Assays. Evidence-Based Complementary and Alternative Medicine. 2012; 519084.

  • Reutens AT, Atkins RC. Epidemiology of Diabetic Nephropathy. Diabetes and The Kidney. 2011; 170: 1-7.

  • Ebadi M. Pharmacodynamic Basis of Herbal Medicine: Alkaloids: Manuka and Fungal Diseases: Flavonoids. New York: CRC Press. 2002.

  • Yadav SK, Nagarathna PKM, Yadav CK. Research article of Evaluation of Immunomodulatory Activity of Dalbergia latifolia on Swiss Albino Mice. IOSR Journal of Pharmacy and Biological Sciences. 2015; 10: 58-64.

  • Yu L-N, Yang X-S, Hua Z, Xie W. Serum Levels of Pro-Inflammatory Cytokines in Diabetic Patients with Peripheral Neuropathic Pain and the Correlation among Them. Zhonghua Yi Xue Za Zhi. 2009; 89(7): 469-471.

  • Navarro-gonzalez JF, Mora-fernandez C. The Role of Inflammatory Cytokines in Diabetic Nephropathy. Journal of the American Society Nephrollogy. 2008; 19: 433-442.

  • Doupis J, Lyons TE, Wu S, Gnardellis C, Dinh T, Veves A. Microvascular Reactivity and Inflammatory Cytokines in Painful and Painless Peripheral Diabetic Neuropathy. The Journal of Clinical Endocrinology & Metabolism. 2009; 94(6): 2157-2163.

  • Muhammad AA, Pauzi NAS, Arulselvan P, Abas F, Fakurazi S. In Vitro Wound Healing Potential and Identification of Bioactive Compounds from Moringa oleifera Lam. BioMed Research International. 2013; 6: 974580.

  • Nieman DC, Henson DA, Davis JM, Angela ME, Jenkins DP, Gross SJ, Carmichael MD, Quindry JC, Dumke CL, Utter AC, McAnulty SR, McAnulty LS, Triplett NT, Mayer EP. Quercetin’s Influence on Exercise-Induced Changes in Plasma Cytokines and Muscle and Leukocyte Cytokine mRNA. Journal of Applied Physiology. 2007; 103(5): 1728-1735.

  • Nair V, Bang WY, Schreckinger E, Andarwulan N, Cisneros-zevallos L. Protective Role of Ternatin Anthocyanins and Quercetin Glycosides from Butter fly Pea (Clitoria ternatea Leguminosae) Blue Flower Petals against Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells. Journal of Agricultural and Food Chemistry. 2015; 63(28): 6355-6365.

  • Nicholson JP, Wolmarans MR, Park GR. The Role of Albumin in Critical Illness. British Journal of Anaesthesia. 2000; 85(4): 599-610.

  • Small DM, Coombes JS, Bennett N, Johnson DW, Gobe GC. Oxidative Stress, Antioxidant Therapies and Chronic Kidney Disease. Nephrology. 2012; 17(4): 311-321.

  • Ruiz S, Pergola PE, Zager RA, Vaziri ND. Targeting the Transcription Factor Nrf2 to Ameliorate Oxidative Stress and Inflammation in Chronic Kidney Disease. Kidney international. 2013; 83(6): 1029-41.

  • Apriasari ML, Ainah Y, Febrianty E, Carabelly AN. Antioxidant Effect of Channa Micropeltes in Diabetic Wound of Oral Mucosa. International Journal of Pharmacology. 2019; 15: 137-143.


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  • Anti-inflammatory Evaluation of Moringa-albumin Combination in Inhibiting Ifn-γ and Tnf-α Expression in Diabetic Mouse Model

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Authors

Noviana Dwi Lestari
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Wahyu Isnia Adharini
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Widodo
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Sri Rahayu
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Hideo Tsuboi
Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550,, Japan
Yoga Dwi Jatmiko
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Muhaimin Rifa’i
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia

Abstract


This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.

Keywords


Albumin, Diabetes Mellitus, IFN-γ. Moringa oleifera, TNF-α,.

References