Research Journal of Pharmacy and Technology

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Anti-inflammatory Evaluation of Moringa-albumin Combination in Inhibiting Ifn-γ and Tnf-α Expression in Diabetic Mouse Model


Affiliations
1 Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
2 Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550,, Japan
     

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This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.

Keywords

Albumin, Diabetes Mellitus, IFN-γ. Moringa oleifera, TNF-α,.
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  • Anti-inflammatory Evaluation of Moringa-albumin Combination in Inhibiting Ifn-γ and Tnf-α Expression in Diabetic Mouse Model

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Authors

Noviana Dwi Lestari
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Wahyu Isnia Adharini
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Widodo
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Sri Rahayu
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Hideo Tsuboi
Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550,, Japan
Yoga Dwi Jatmiko
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia
Muhaimin Rifa’i
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java,, Indonesia

Abstract


This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.

Keywords


Albumin, Diabetes Mellitus, IFN-γ. Moringa oleifera, TNF-α,.

References