Research Journal of Pharmacy and Technology

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Uv-visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin


Affiliations
1 MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
2 MM School of Pharmacy, MM University, Sadopur, Ambala, Haryana,, India
3 Maharishi Markandeshwar University, Solan, HP,, India
     

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Background: Curcumin shows degradation in the solution of high pH. There is a need for development of a method which can estimate the drug release precisely and accurately. The UV spectroscopy due to its simplicity and specificity is the most popular for method development for the determination of drugs. Objective: The present study was aimed to develop the UV-Visible spectrophotometric method for the assessment of curcumin and tetrahydrocurcumin (THC) using a stable solvent system. Method: The solubility of curcumin and tetrahydrocurcumin was determined at various physiological pH levels. Non-ionic surfactant, tween 80 was used with selected media to avoid the degradation of drugs. The selected solvent system was further validated according to guidelines of the international conference on harmonization (ICH) and the analytical parameters like linearity, veracity, sensitivity and accuracy were studied. Results: The solvent systems Phosphate buffer of pH 6.8+2% tween 80 and Phosphate buffer of pH 6.8+1% tween 80 were found optimum for estimation of curcumin and THC respectively. All the validation parameters were found within the range for developed methods. Conclusion: The proposed method is very simple and can be used for routine quality control testing of curcumin and tetrahydrocurcumin.

Keywords

Curcumin, Tetrahydrocurcumin, Phosphate buffer pH 6.8, Tween 80, UV-Visible spectrophotometric method development.
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  • Krishna Veni N, Meyyanathan SN, Rajinikanth BR, Elango K. A liquid chromatography method for the simultaneous determination of curcumin and piperine in food products using diode array detection. Asian J. Research Chem. 2009; 2(2): 115-118.

  • Wandhare MD, Deokate UA, Khadabadi SS, Hadke SP, Sawarkar HA. Comparative Estimation of Curcumin Content from Marketed Herbal Anti Rheumatic Tablets Formulation. Asian J. Research Chem., 2009; 2(3): 340-3.

  • Nair TS, Meghana R, Shlini P. Antimicrobial Activity of the protein fraction obtained in the extraction of Curcumin. Asian J. Research Chem., 2019;12(4): 199-202.

  • Kollipara RK, Tallapaneni V, Sanapalli BK, Kumar GV, Karri VV. Curcumin loaded ethosomal vesicular drug delivery system for the treatment of melanoma skin cancer. Research J. Pharm. and Tech., 2019;12(4): 1783-92.

  • Sweetha G, Sangeetha B, Prabhu S. A review on curcumin nanoparticles and its controlled delivery to treat degenerative diseases. Asian J. Pharm. Tech., 2013; 28;3(4): 218-22.

  • Holder GM, Plummer JL, Ryan AJ. The metabolism and excretion of curcumin (1, 7-bis-(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3, 5-dione) in the rat. Xenobiotica. 1978;8(12):761-8.

  • Mahal A, Wu P, Jiang ZH, Wei X. Schiff bases of tetrahydrocurcumin as potential anticancer agents. ChemistrySelect., 2019;4(1):366-9.

  • Xi J, Luo X, Wang Y, Li J, Guo L, Wu G, Li Q. Tetrahydrocurcumin protects against spinal cord injury and inhibits the oxidative stress response by regulating FOXO4 in model rats. Exp. Ther. Med., 2019;18(5):3681-7.

  • Murugan P, Pari L. Influence of tetrahydrocurcumin on erythrocyte membrane bound enzymes and antioxidant status in experimental type 2 diabetic rats. J. Ethnopharmacol., 2007;113(3):479-86.

  • Kimura S, Kiriyama A, Araki K, Yoshizumi M, Enomura M, Inoue D, et al. Novel strategy for improving the bioavailability of curcumin based on a new membrane transport mechanism that directly involves solid particles. Eur J Pharm and Biopharm., 2018;122:1-5.

  • Pari L, Murugan P. Changes in glycoprotein components in streptozotocin-nicotinamide induced type 2 diabetes: influence of tetrahydrocurcumin from Curcuma longa. Plant Food Hum. Nut., 2007 1;62(1):25-9.

  • Jayandran M, Haneefa MM, Balasubramanian V. Synthesis, Characterization and antimicrobial activities of turmeric curcumin and curcumin stabilized zinc nanoparticles-A green approach. Research J. Pharm. and Tech., 2015;8(4):445.

  • Rramaswamy R, Mani G, Venkatachalam S, Yasam RV, Rajendran JB, Tae JH. Preparation and characterization of tetrahydrocurcumin-loaded cellulose acetate phthalate/polyethylene glycol electrospun nanofibers. AAPS PharmSciTech., 2018;19(7):3000-8.

  • Rapalli VK, Kaul V, Gorantla S, Waghule T, Dubey SK, Pandey MM, et al. UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: method development, in-vitro and ex-vivo applications in topical delivery. Spectrochimica Acta A,. 2020;224:117392.

  • Mondal S, Ghosh S, Moulik SP. Stability of curcumin in different solvent and solution media: UV–visible and steady-state fluorescence spectral study. J. Photochem. Photobiol. B, Biol., 2016;158:212-8.

  • Aggarwal BB, Deb L, Prasad S. Curcumin differs from tetrahydrocurcumin for molecular targets, signaling pathways and cellular responses. Molecules., 2015;20(1):185-205.

  • Majumder KK, Sharma JB, Kumar M, Bhatt S, Saini V. Development and Validation of UV-Visible Spectrophotometric Method for The Estimation of Curcumin in Bulk and Pharmaceutical Formulation. Pharmacophores., 2020; 10(1):115-21.

  • Martínez-Guerra J, Palomar-Pardavé M, Romero-Romo M, Corona-Avendaño S, Rojas-Hernández A, Ramírez-Silva MT. New insights on the Chemical Stability of Curcumin in Aqueous Media at Different pH: Influence of the Experimental Conditions. Int. J. Electrochem. Sci., 2019;14:5373-85.

  • He P, Yan H, Zhao J, Gou M, Li X. An evaluation of the wound healing potential of tetrahydrocurcumin-loaded MPEG-PLA nanoparticles. J. Biomater. Appl., 2019;34(3):315-25.

  • Ajay S, Harita D, Tarique M, Amin P. Solubility and dissolution rate enhancement of curcumin using kollidon VA64 by solid dispersion technique. Int J Pharm Tech Res., 2012; 4:1055-64.

  • ICH-Guidelines Q2(R1). Validation of Analytical Procedures: Text and Methodology. Geneva, Switzerland: 2005.

  • Pramod K, Ansari SH, Ali J. UV Spectrophotometric Method for the Quantification of Eugenol during in Vitro Release Studies. Asian J. Pharm. Ana., 2013;3(3):86-9.

  • Patidar M, Gopkumar P, Sridevi G, Behera CC, Pillai S. Development and Validation of RP-HPLC Method for Simultaneous Determination of Resveratrol and Curcumin in Pure Form. Research J. Pharm. and Tech., 2013;6(9):990-2.

  • Tang Y, Fields C. A UHPLC-UV Method development and validation for determining kavalactones and flavokavains in Piper methysticum (Kava). Molecules., 2019;24(7):1245.

  • Nagarnaik M, Sarjoshi A, Bodkhe A, Khanal B, Pise M, Pandya G. Characterization of active constituents in Turmeric powder and validation of method for curcumin in samples. Asian J. Research Chem., 2015;8(10):643-7.

  • Sahu S, Kumari K, Muduli NR, Moharana AK. Development of UV Spectrophotometry Absorption correction method for estimation of Curcumin and Aspirin from Bulk. Research J. Pharm. and Tech., 2019;12(10):4857-60.

  • Rahman SM, Telny TC, Ravi TK, Kuppusamy S. Role of surfactant and pH in dissolution of curcumin. Indian J. Pharma. Sci., 2009;71(2):139.

  • Trivedi MK, Panda P, Sethi KK, Gangwar M, Mondal SC, Jana S. Solid and liquid state characterization of tetrahydrocurcumin using XRPD, FT-IR, DSC, TGA, LC-MS, GC-MS, and NMR and its biological activities. J. Pharm. Anal., 2020;10(4):334-45.


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  • Uv-visible Spectrophotometric Method Development and Validation for the Estimation of Curcumin and Tetrahydrocurcumin

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Authors

Jai Bharti Sharma
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
Shailendra Bhatt
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India
Vipin Saini
MM School of Pharmacy, MM University, Sadopur, Ambala, Haryana,, India
Rupesh K. Gautam
Maharishi Markandeshwar University, Solan, HP,, India
Manish Kumar
MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana,, India

Abstract


Background: Curcumin shows degradation in the solution of high pH. There is a need for development of a method which can estimate the drug release precisely and accurately. The UV spectroscopy due to its simplicity and specificity is the most popular for method development for the determination of drugs. Objective: The present study was aimed to develop the UV-Visible spectrophotometric method for the assessment of curcumin and tetrahydrocurcumin (THC) using a stable solvent system. Method: The solubility of curcumin and tetrahydrocurcumin was determined at various physiological pH levels. Non-ionic surfactant, tween 80 was used with selected media to avoid the degradation of drugs. The selected solvent system was further validated according to guidelines of the international conference on harmonization (ICH) and the analytical parameters like linearity, veracity, sensitivity and accuracy were studied. Results: The solvent systems Phosphate buffer of pH 6.8+2% tween 80 and Phosphate buffer of pH 6.8+1% tween 80 were found optimum for estimation of curcumin and THC respectively. All the validation parameters were found within the range for developed methods. Conclusion: The proposed method is very simple and can be used for routine quality control testing of curcumin and tetrahydrocurcumin.

Keywords


Curcumin, Tetrahydrocurcumin, Phosphate buffer pH 6.8, Tween 80, UV-Visible spectrophotometric method development.

References