Research Journal of Pharmacy and Technology

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Green Synthesis and Standardization of Biologically Synthesized Silver Nanoparticles


Affiliations
1 Assistant Professor, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
2 Director, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
3 Professor, L.J.Institute of Applied Sciences, L.J.K Campus, S.G. Highway, Ahmedabad., India
     

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The biological silver nanoparticle was synthesized extracellularly by using Musa balbisiana. The leaf extract has been used as reducing agent in the process of nanoparticles synthesis. In 50 ml leaf extract volume of AgNO3 stock solution was added to make finally the concentration as 1 mM of AgNO3 and allowed to shake in an incubator for several hours in the dark. The changed color was considered as the primary indication of nanoparticles formation. It was indicated that stable and 20-40nm roughly spherical shaped silver nanoparticles was formed. To standardize the nanoparticles biosynthesis different physical parameters like substrate concentration (0-8 mM), pH of range 5 to 12, temperature range of 5 to 500°C, incubation time range of 0 to 120 hrs and salinity of 0.1-1.0% were investigated. It was reported that 4mM AgNO3 conc, pH of 9, temperature 300°C, incubation time 72h and 0.2% salinity were found to be optimum for the synthesis and stability of the silver nanoparticles.

Keywords

Silver nanoparticles, Musa balbisiana, Optimising parameters, Standardization
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  • Green Synthesis and Standardization of Biologically Synthesized Silver Nanoparticles

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Authors

Dave Archit1,
Assistant Professor, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
Sharma Kuldeep
Director, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
Chandramauly R. Sharma
Professor, L.J.Institute of Applied Sciences, L.J.K Campus, S.G. Highway, Ahmedabad., India

Abstract


The biological silver nanoparticle was synthesized extracellularly by using Musa balbisiana. The leaf extract has been used as reducing agent in the process of nanoparticles synthesis. In 50 ml leaf extract volume of AgNO3 stock solution was added to make finally the concentration as 1 mM of AgNO3 and allowed to shake in an incubator for several hours in the dark. The changed color was considered as the primary indication of nanoparticles formation. It was indicated that stable and 20-40nm roughly spherical shaped silver nanoparticles was formed. To standardize the nanoparticles biosynthesis different physical parameters like substrate concentration (0-8 mM), pH of range 5 to 12, temperature range of 5 to 500°C, incubation time range of 0 to 120 hrs and salinity of 0.1-1.0% were investigated. It was reported that 4mM AgNO3 conc, pH of 9, temperature 300°C, incubation time 72h and 0.2% salinity were found to be optimum for the synthesis and stability of the silver nanoparticles.

Keywords


Silver nanoparticles, Musa balbisiana, Optimising parameters, Standardization

References