Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Green Synthesis and Standardization of Biologically Synthesized Silver Nanoparticles


Affiliations
1 Assistant Professor, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
2 Director, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
3 Professor, L.J.Institute of Applied Sciences, L.J.K Campus, S.G. Highway, Ahmedabad., India
     

   Subscribe/Renew Journal


The biological silver nanoparticle was synthesized extracellularly by using Musa balbisiana. The leaf extract has been used as reducing agent in the process of nanoparticles synthesis. In 50 ml leaf extract volume of AgNO3 stock solution was added to make finally the concentration as 1 mM of AgNO3 and allowed to shake in an incubator for several hours in the dark. The changed color was considered as the primary indication of nanoparticles formation. It was indicated that stable and 20-40nm roughly spherical shaped silver nanoparticles was formed. To standardize the nanoparticles biosynthesis different physical parameters like substrate concentration (0-8 mM), pH of range 5 to 12, temperature range of 5 to 500°C, incubation time range of 0 to 120 hrs and salinity of 0.1-1.0% were investigated. It was reported that 4mM AgNO3 conc, pH of 9, temperature 300°C, incubation time 72h and 0.2% salinity were found to be optimum for the synthesis and stability of the silver nanoparticles.

Keywords

Silver nanoparticles, Musa balbisiana, Optimising parameters, Standardization
Subscription Login to verify subscription
User
Notifications
Font Size


  • Pranati T, Anitha R, Rajeshkumar S, Lakshmi T. Preparation of Silver nanoparticles using Nutmeg oleoresin and its Antimicrobial activity against Oral pathogens. Research Journal of Pharmacy and Technology. 2019;12(6):2799-803.

  • Karande KM, Gawade SP. Synthesis of Nanosilver and its Comparative Evaluation of Cytotoxic Activity. Research Journal of Pharmacy and Technology. 2020;13(2):659-63.

  • Patil RY, Patil SA, Chivate ND, Patil YN. Herbal drug nanoparticles: advancements in herbal treatment. Research Journal of Pharmacy and Technology. 2018;11(1):421-6.

  • Rajakumari K. Nanotherapy for Cancer-A Review. Research Journal of Pharmacy and Technology. 2020;13(3):1575-9.

  • Thyagarajan R, Namasivayam S, Narendrakumar G, Singh V, Samydurai S. Evaluation of in Vitro Drug Controlled Release of Biocompatible Metallic and Non Metallic Nanoparticles Incorporated Anti Bacterial Antibiotics and Their Anti Biofilm Activity Against E. coli. Research Journal of Pharmacy and Technology. 2015;8(3):316-9.

  • Usha AL, Kumari MK, Rani ER, Bhavani AK. A Novel Technique for Intra Transdermal Delivery of Drugs–Coated Polymeric Needles. Asian Journal of Pharmacy and Technology. 2020 Nov 18;10(4):289-95.

  • Maikifi AS, Damodharan N. Nanodiamonds: Synthesis, Properties, Toxicities and an update on its effective uses in Anticancer Drugs Deliveries. Research Journal of Pharmacy and Technology. 2020 Nov 1;13(11):5529-33.

  • Kengar MD, Jadhav AA, Kumbhar SB, Jadhav RP. A Review on Nanoparticles and its Application. Asian Journal of Pharmacy and Technology. 2019;9(2):115-24.

  • Sadhasivam J, Sugumaran A, Narayanaswamy D. Nano Sponges: A Potential Drug Delivery Approach. Research Journal of Pharmacy and Technology. 2020 Jul 1;13(7):3442-8.

  • Sadhasivam J, Sugumaran A, Narayanaswamy D. Nano Sponges: A Potential Drug Delivery Approach. Research Journal of Pharmacy and Technology. 2020 Jul 1;13(7):3442-8.

  • Saxena A, Tripathi RM, Singh RP. Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Dig J Nanomater Bios. 2010 Jun 1;5(2):427-32.


Abstract Views: 115

PDF Views: 0




  • Green Synthesis and Standardization of Biologically Synthesized Silver Nanoparticles

Abstract Views: 115  |  PDF Views: 0

Authors

Dave Archit1,
Assistant Professor, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
Sharma Kuldeep
Director, Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad, Gujarat-380058., India
Chandramauly R. Sharma
Professor, L.J.Institute of Applied Sciences, L.J.K Campus, S.G. Highway, Ahmedabad., India

Abstract


The biological silver nanoparticle was synthesized extracellularly by using Musa balbisiana. The leaf extract has been used as reducing agent in the process of nanoparticles synthesis. In 50 ml leaf extract volume of AgNO3 stock solution was added to make finally the concentration as 1 mM of AgNO3 and allowed to shake in an incubator for several hours in the dark. The changed color was considered as the primary indication of nanoparticles formation. It was indicated that stable and 20-40nm roughly spherical shaped silver nanoparticles was formed. To standardize the nanoparticles biosynthesis different physical parameters like substrate concentration (0-8 mM), pH of range 5 to 12, temperature range of 5 to 500°C, incubation time range of 0 to 120 hrs and salinity of 0.1-1.0% were investigated. It was reported that 4mM AgNO3 conc, pH of 9, temperature 300°C, incubation time 72h and 0.2% salinity were found to be optimum for the synthesis and stability of the silver nanoparticles.

Keywords


Silver nanoparticles, Musa balbisiana, Optimising parameters, Standardization

References