Research Journal of Pharmacy and Technology

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Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass Spectroscopy (Lc-ms)


Affiliations
1 K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
2 Research Scholar, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh-522 502., India
     

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Objective: Highly responsive simple Liquid-liquid Extraction Glipizide development using deuterated Glipizide and Sitagliptin LC-MS/MS Glipizide for gradation of Glipizide and Sitagliptin in rat plasma Glipizide: The chromatographic condition involves, isocratic mode using waters X-Bridge C18 3.5μ (150x4.6mm) column. Mobile phase 0.1% orthophosphoric acid and Acetonitrile in the ratio (80:20) was used and detection was carried out in a positive mode of electrospray ionization by using MS. Results: Glipizide has been validated, the linearity was observed in the range 10-150 ng/ml and 0.1-1.5 ng/ml for Glipizide and Sitagliptin respectively. For intraday and inter-day precision %RSD values were found to be within the acceptable limits. Recovery studies for Glipizide and Sitagliptin obtained was 99.5% and 98.6% respectively. The battery of stability studies like bench-top, Autosampler stability, freeze-thaw, and long term stability was performed. Conclusion: Highly responsive simple liquid chromatography tandem mass spectroscopy assay Glipizidewas developed and witnessed for the gradation of Glipizideand Sitagliptin in rat plasma, the developed Glipizidewas applied to pharmacokinetic studies.

Keywords

LCMS, Glipizide, Sitagliptin, Glipizidevalidation, pharmacokinetic study.
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  • Badian M, Korn A, Lehr KH, Malerczyk V, Waldhausl W (1994) Drug Metab Drug Interact 11:331, doi: 10.1515/dmdi.1994.11.4.331

  • Physicians Desk Reference (PDR) (2001) Medicinal Economics Inc., 55th edn. Montvale, NJ, p 68, doi: 10.1515/dmdi.1994.11.5.321

  • Nunez M, Ferguson JE, Machacek D, Jacob G, Oda RP, Lawson GM, Landers JP, Determination of Glimepiride in human Plasma by Liquid chromatography -Electroscopy ionization tandem masss spectrometry, 109-114, https://doi.org/10.5012/bkcs.2004.25.1.109.

  • Roche ME, Oda RP, Lawson GM, Land- ers JP, Capillary electrophoretic detection of metabolites in the urine of patients receiving hypoglycemic drug therapy 18:1865–1874, doi: 10.1002/elps.1150181024.

  • Drummer OH, Kotsos A, McIntyree IM (1993),A Class independent drug screens using array technology using a array detector, J Anal Toxicol, 17:225–229, doi: 10.1515/dmdi.1994.11.5.321

  • Lo DS, Chao TC, Ng Ong SE, Yao YJ, Koh TH (1997) Forensic Sci Int 90:205– 217, http://dx.doi.org/10.1016/s0379-0738(97)00170-9

  • Lehr K, Damm PJ (1990), Determination of Glimepiride in Human Plasma by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry Chromatogra- phy 526:497–505. https://doi.org/10.5012/bkcs.2004.25.1.109

  • FDA Guidance for Industry, Bioanalytical Method Validation, May 2001

  • Salem II, Idrees J, Al Tamimi JI (2004), Determination of glimepiride in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry J Chromatogr B 799(1):103–109, https://doi.org/10.1016/j.jchromb.2003.10.024


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  • Rapid Quantitative Estimation of Glipizide and Sitagliptin in Rat Plasma by Liquid Chromatography and Mass Spectroscopy (Lc-ms)

Abstract Views: 276  |  PDF Views: 0

Authors

A. Anka Rao
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
K. Pavan Kumar
Research Scholar, K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh-522 502., India
A. Narayana Rao
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
Narender Malothu
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
B. Prassana Kumar Desu
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India
Naga Raju Bandaru
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh - 522 502., India

Abstract


Objective: Highly responsive simple Liquid-liquid Extraction Glipizide development using deuterated Glipizide and Sitagliptin LC-MS/MS Glipizide for gradation of Glipizide and Sitagliptin in rat plasma Glipizide: The chromatographic condition involves, isocratic mode using waters X-Bridge C18 3.5μ (150x4.6mm) column. Mobile phase 0.1% orthophosphoric acid and Acetonitrile in the ratio (80:20) was used and detection was carried out in a positive mode of electrospray ionization by using MS. Results: Glipizide has been validated, the linearity was observed in the range 10-150 ng/ml and 0.1-1.5 ng/ml for Glipizide and Sitagliptin respectively. For intraday and inter-day precision %RSD values were found to be within the acceptable limits. Recovery studies for Glipizide and Sitagliptin obtained was 99.5% and 98.6% respectively. The battery of stability studies like bench-top, Autosampler stability, freeze-thaw, and long term stability was performed. Conclusion: Highly responsive simple liquid chromatography tandem mass spectroscopy assay Glipizidewas developed and witnessed for the gradation of Glipizideand Sitagliptin in rat plasma, the developed Glipizidewas applied to pharmacokinetic studies.

Keywords


LCMS, Glipizide, Sitagliptin, Glipizidevalidation, pharmacokinetic study.

References