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RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)


Affiliations
1 CCS Haryana Agricultural University, Hisar-125004, India
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, India
     

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Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.

Keywords

Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPD
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About The Authors

R.S. Dhillon
CCS Haryana Agricultural University, Hisar-125004
India

R.P. Saharan
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf
Germany

M. Jattan
CCS Haryana Agricultural University, Hisar-125004
India

T. Rani
CCS Haryana Agricultural University, Hisar-125004
India

Charan Singh
Central Soil and Water Conservation Research and Training Institute, Dehradun
India

George Von Wuehlisch
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf
Germany


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  • Achten, W.M.J., Verchot, L., Franken, Y.J., Mathijs, E., Singh, V.P., Aerts, R. and Muys, B. (2008). Jatropha bio-diesel production and use. Biomass Bioener., 32: 1063-1084.

  • Akhaury, K.D.N. and Singh, A.K. (1993). Effect of Gamma-rays on the seed output of Vicia species. Neo Botanica, 1 (1&2) 63–67.

  • Basha, S.D. and Sujatha, M. (2007). Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica, 156: 375-386.

  • Bhagwat, A., Krishna, T.G. and Bhatia, C.R.. (1997). RAPD analysis of induced mutants of groundnut (Arachis hypogaea L.). J. Genet., 76(3): 201-208.

  • Charbaji, T. and Nabulsi, I. (1999). Effect of low doses of gamma irradiation on in vitro growth of grapevine. Plant Cell Tissue Organ Cult., 57:129–132.

  • Chauhan, Y.S. (1978). Gamma rays-induced variation in the development of S. khasianum Clarke. J. Indian Bot. Soc., 57: 347–352.

  • Dhakshanamoorthy, D., Selvaraj, R. and Chidambaram, A.L.A. (2011). Induced mutagenesis in Jatropha curcas L. using gamma rays and detection of DNA polymorphism through RAPD marker. C. R. Biologies, 334: 24–30.

  • Dhillon, R.S., Hooda, M.S., Jattan, M., Chawla, V., Bhardwaj, M. and Goyal, S.C. (2009). Development and molecular characterization of interspecific hybrids of Jatropha curcas J. integerrima. Indian J. of Biotechnol., 8: 384-390.

  • Fritsch, P., Hanson, M.A., Spore, C.D. Pack, P.E. and Riseberg, H. (1993). Constancy of RAPD primer amplification strength among distantly related taxa of flowering plants. Plant Mol. Biol. Rep., 11: 10-20.

  • Gunckel, J.E. and Sparrow,A.H. (1991). Ionizing radiation: biochemical, physiological and morphological aspects of their effects on plants. W. Ruland (Ed.), Plant Physiolo. XVI, Springer Verlag, Berlin. 555–611.

  • Khawale, R.N., Yerramilli, V. and Singh, S.K. (2007). Molecular marker-assisted selection of in vitro chemical mutagen-induced grapevine mutants. Curr. Sci., 92: 1056 -1060.

  • Kumar, A. and Mishra, M.N. (2004). Effect of gamma-rays, EMS and NMU on germination, seedling vigour, pollen viability and plant survival in M and M generation of Okra (Abelmoschus esculentus (L.) Moench). Adv. Plant Sci., 17:295–297. 1 2

  • Kumar, A. and Sharma, S. (2008). An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): A review. Industrial Crops and Products, 28: 1-10.

  • Kumar, R.S., Parthiban, K.T., Hemalatha, P., Kalaiselvi, T. and Rao, M.G. (2009). Investigation on cross-compatibility barriers in the biofuel crop Jatropha curcas L. with wild Jatropha species. Crop Sci., 49: 1667-1674.

  • Kumar, S., Prasad, K.V. and Choudhary, M.L. (2006). Detection of genetic variability among Chrysanthemum radiomutants using RAPD markers. Curr. Sci., 90: 1103-1118.

  • Lilley, R. (2008). NZ airline flies jetliner partly run on biofuel. New Zealand Herald (http://www.google.com/ hostednews/ap/article/ALeqM5gJ5gWEPiD5VL1BQKcNSbHthbCkAD95DC5R02).

  • Melki, M. and Marouani, A. (2009). Effects of gamma rays irradiation on seed germination and growth of hard wheat. Environ. Chem. Lett. doi:10.1007/s10311-009-0222-1.

  • Murry, M. and Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nuc. Aci. Res., 8: 4321-4325.

  • Panse, V.G. and Sukhatme, P.V. (1978). Statistical methods for agricultural workers, New Delhi. ICAR Publication.

  • Popluechai, S., Breviario, D., Sujatha, M., Makkar, H.P.S., Raorane, M., Reddy, A.R., Palchetti, E., Gatehouse, A.M.R., Syers, J.K., Donnell, A.G.O. and Kohli, A. (2009). Narrow genetic and apparent phenetic diversity in Jatropha curcas: initial success with generating low phorbol ester interspecific hybrids. Nature Precedings DOI hdl:10101/npre.2009.2782.1

  • Rohlf, F.J. (1990). NTSYS-PC Numerical Taxonomy and Multivariate Analysis System. Version 1.60 (Exeter Publications, New York).

  • Ruminska, J.L., Zalewska, M. and Sadoch, Z. (2004). Radio-mutants of Chrysanthemum (Dendranthema grandiflora Tzvelev) of the lady group: RAPD analysis of the genetic diversity. Plant Breed., 123: 290-293.

  • Sambrook, J., Frietch, E.F. and Maniatis, T. (1989). Molecular Cloning: a laboratory manual 2nd ed. Cold Spring Harbour Laboratory, New York.

  • Selvi, B.S., Ponnuswami, V. and Sumathi, T. (2007). Identification of DNA polymorphism induced by gamma ray irradiation in Amla (Emblica officinalis Gaertn.) grafts of V1 M1 and V2 M1 generation. J. Appl. Sci. Res., 3: 1933-1935.

  • Songsri, P., Suriharn, B. Sanitchon, J., Srisawangwong, S. and Kesmala, T. (2011). Effect of gamma radiation on germination and growth characterstics of physic nut (Jatropha curcas L.). J. of Biol. Sci., 11: 268-274.

  • Tanya, P., Taeprayoon, P., Hadkam, Y. and Srinives, P. (2011). Genetic diversity among jatropha and jatropha related species based on ISSR markers. Plant Mol. Biol. Repor., 29: 252-264.

  • Taylor, F.G. (1968). Some effect of acute gamma radiation in giant sequoia seedlings. Radiat. Bot., 8: 67–70.

  • Teng, N., Chen, F., Jiang, Z., Fang, W. and Chen, T. (2008). Detection of genetic variation by RAPD among Chrysanthemum plantlets regenerated from irradiated calli. Acta Hort., 766: 413-420.

  • Thapa, C.B. (1999). Effect of acute exposure of gamma rays on seed germination of Pinus kesiya Gord and P. wallichiana A.B. Jacks, Botanica Orientalis. J. Plant Sci., 120–121.

  • Thapa, C.B. (2004). Effect of acute exposure of gamma rays on seed germination and seedling growth of Pinus kesiya Gord and P. wallichiana A.B. Jacks. Our Nature, 2: 13–17. http://www.nepjol.info/index.php/ON/ article/view Article/318.

  • Zaka, R., Chenal, C. and Misset, M.T. (2004). Effect of low doses of short-term gamma radiation on growth and development through two generations of Pisum sativum. Sci. Total Environ., 320:121–129. doi:10.1016/j.scitotenv.2003.08.010.


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  • RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)

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Authors

R.S. Dhillon
CCS Haryana Agricultural University, Hisar-125004, India
R.P. Saharan
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany
M. Jattan
CCS Haryana Agricultural University, Hisar-125004, India
T. Rani
CCS Haryana Agricultural University, Hisar-125004, India
Charan Singh
Central Soil and Water Conservation Research and Training Institute, Dehradun, India
George Von Wuehlisch
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany

Abstract


Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.

Keywords


Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPD

References