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RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)


Affiliations
1 CCS Haryana Agricultural University, Hisar-125004, India
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, India
     

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Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.

Keywords

Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPD
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About The Authors

R.S. Dhillon
CCS Haryana Agricultural University, Hisar-125004
India

R.P. Saharan
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf
Germany

M. Jattan
CCS Haryana Agricultural University, Hisar-125004
India

T. Rani
CCS Haryana Agricultural University, Hisar-125004
India

Charan Singh
Central Soil and Water Conservation Research and Training Institute, Dehradun
India

George Von Wuehlisch
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf
Germany


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  • RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)

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Authors

R.S. Dhillon
CCS Haryana Agricultural University, Hisar-125004, India
R.P. Saharan
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany
M. Jattan
CCS Haryana Agricultural University, Hisar-125004, India
T. Rani
CCS Haryana Agricultural University, Hisar-125004, India
Charan Singh
Central Soil and Water Conservation Research and Training Institute, Dehradun, India
George Von Wuehlisch
Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, Germany

Abstract


Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.

Keywords


Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPD

References