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Analysis of Edible Fruits against Glycolytic Enzymes and Glycation:In vitro Approaches with in silico Validation


Affiliations
1 Laboratory of Foods and Nutrition, Post Graduate Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Anand (Gujarat), India
2 UGC-Centre of Advanced Study, Post Graduate Department of Biosciences, Sardar Patel University, Satellite Campus, Bakrol, Anand (Gujarat), India
3 Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Anand (Gujarat), India
     

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Diabetes is the most quotidian endocrine disorder and one of the fastest growing non-communicable diseases around the globe.Commonly consumed six fruits were investigated to determine their therapeutic potential to inhibitoxidation, key glycolytic enzymes and glycation activity which has relevance in the management of hyperglycemia and type II diabetes. The in vitro analysis revealed that amla fruit showed maximum total phenols and total antioxidant capacity among all the six fruits. Amla fruit exhibited potent inhibition for both alpha amylase and alpha glucosidase enzyme activity than the positive control acarbose. The IC50value of alpha amylase inhibition and alpha glucosidase inhibition in amla was found to be high among all the fruits. Further, amla fruit phenolic compound (gallic acid) confirmed better in silico enzyme inhibitory action with alpha glucosidase with a binding energy of -6.21kcal/mol than alpha amylase. In antiglycation activity amla and mango fruits showed potent inhibition. Pearson correlation results showed a strong correlation (p<0.01) between total phenol with flavonoids, total antioxidant capacity and antiglycation activities.The results obtained in this study showed that amla and mango had potent potential for the management of hyperglycemia, diabetes and the related condition of oxidative stress. Hence, these fruits can be prescribed to treat diabetes in safest way by incorporating them in natural medications.

Keywords

Fruits, Alpha Amylase, Alpha Glucosidase, Antiglycation, Antioxidant, In silico.
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  • Adisakwattana, S., Lerdsuwankij, O., Poputtachai, U., Minipun, A. and Suparpprom, C. (2011). Inhibitory activity of cinnamon bark species and their combination effect with acarbose against intestinal -glucosidase and pancreatic α-amylase. Plant Foods for Human Nutr., 66 (2) : 143-148.

  • Babu, T. H., Rao, V. R. S., Tiwari, A. K., Babu, K. S., Srinivas, P. V., Ali, A. Z. and Rao, J.M. (2008). Synthesis and biological evaluation of novel 8-aminomethylated oroxylinA analogues as -glucosidase inhibitors. Bioorganic &MedicinalChem. Letters, 18(5): 1659-1662.

  • Baker, J. R., Metcalf, P. A., Johnson, R., Newman, D. and Rietz, P. (1985). Use of protein-based standards in automated colorimetric determinations of fructosamine in serum. ClinicalChemistry, 31(9) : 1550-1554.

  • Benzie, I. F. and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochem., 239(1): 70-76.

  • Brand-Williams, W., Cuvelier, M. E. and Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science & Technol., 28(1): 25 - 30.

  • Brings, S., Fleming, T., Freichel, M., Muckenthaler, M., Herzig, S. and Nawroth, P. (2017). Dicarbonyls and advanced glycation end-products in the development of diabetic complications and targets for intervention. International J. Molecular Sci., 18(5): 984.

  • Chakrabarti, R. and Rajagopalan, R. (2002). Diabetes and insulin resistance associated disorders: disease and the therapy. Current Science, 83 (12): 1533-1538.

  • Choudhary, D. K. and Mishra, A. (2017). In vitro and in silico interaction of porcine α-amylase with Viciafaba crude seed extract and evaluation of antidiabetic activity. Bioengineered, 8 (4) : 393-403.

  • Fred-Jaiyesimi, A., Kio, A. and Richard, W. (2009). α-Amylase inhibitory effect of 3 β-olean-12-en-3-yl (9Z)-hexadec-9enoate isolated from Spondiasmombin leaf. Food Chemistry, 116(1), 285-288.

  • Ganogpichayagrai, A., Palanuvej, C. and Ruangrungsi, N.(2017). Antidiabetic and anticancer activities of Mangifera indica cv. OKRONG leaves. J.Adv. Pharmaceut. Technol. &Res., 8 (1) : 19.

  • Gao, H., Nishioka, T., Kawabata, J. and Kasai, T. (2004). Structure-activity relationships for -glucosidase inhibition of baicalein, 5, 6, 7-trihydroxyflavone: the effect of A-ring substitution. Bioscience, Biotechnology & Biochemistry, 68 (2) : 369-375.

  • Gao, H. and Kawabata, J. (2005). - glucosidase inhibition of 6- hydroxyflavones. Part 3: Synthesis and evaluation of 2, 3, 4-trihydroxybenzoyl-containing flavonoid analogs and 6-aminoflavones as -glucosidase inhibitors. Bioorganic &Medicinal Chemistry, 13(5) : 1661-1671.

  • Gilson, M. K. and Zhou, H. X. (2007). Calculation of proteinligand binding affinities. Annu. Rev. Biophys. Biomol. Struct., 36 : 21-42.

  • Guariguata, L., Whiting, D. R., Hambleton, I., Beagley, J., Linnenkamp, U. and Shaw, J. E. (2014). Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res. &Clinical Practice, 103(2) : 137-149.

  • Gu, Jenny, Strauss, Clara, Bond, Rod and Cavanagh, Kate (2015). How do mindfulness-based cognitive therapy and mindfulness-based stress reduction improve mental health and wellbeing? A systematic review and meta-analysis of mediation studies. Clinical Psychology Review, 37: 1– 12.

  • Jhong, C. H., Riyaphan, J., Lin, S. H., Chia, Y. C. and Weng, C. F. (2015). Screening alpha glucosidase and alpha amylase inhibitors from natural compounds by molecular docking in silico. Biofactors, 41(4) : 242-251.

  • Kaewnarin, K., Niamsup, H., Shank, L. and Rakariyatham, N. (2014). Antioxidant and antiglycation activities of some edible and medicinal plants. Chiang Mai. J. Sci., 41(1) : 105-116.

  • Kimmel, B. and Inzucchi, S. E. (2005). Oral agents for type 2 diabetes: an update. Clinical Diabetes, 23(2) : 64-76.

  • Kitchen, D. B., Decornez, H., Furr, J. R. and Bajorath, J. (2004). Docking and scoring in virtual screening for drug discovery: methods and applications. Nature Rev. Drug Discovery, 3(11) : 935.

  • Ku, S., You, H. J. and Ji, G. E. (2009). Enhancement of antitumorigenic polysaccharide production, adhesion and branch formation of Bifidobacteriumbifidum BGN4 by phytic acid. Food Sci.& Biotechnol., 18(3) : 749-754.

  • Laulloo, S.J., Bhowon, M.G., Chua, L.S. and Gaungoo, H. (2018). Phytochemical screening and antioxidant properties of phyllanthusemblica from mauritius. Chemistry of Natural Compounds, 54 (1) : 50-55.

  • Liu, X., Zhao, M., Wang, J., Yang, B. and Jiang, Y. (2008). Antioxidant activity of methanolic extract of emblica fruit (Phyllanthusemblica L.) from six regions in China. J.Food Composition & Analysis, 21(3) : 219-228.

  • Li, X., Zheng, T., Sang, S. and Lv, L. (2014). Quercetin inhibits advanced glycation end product formation by trapping methylglyoxal and glyoxal. J. Agric. & Food Chem., 62 (50) : 12152-12158.

  • Lo, C. Y., Hsiao, W. T. and Chen, X. Y. (2011). Efficiency of trapping methylglyoxal by phenols and phenolic acids. J. Food Sci., 76(3) : 90-96.

  • Muthuraman, A., Sood, S. and Singla, S. K. (2011). The antiinflammatory potential of phenolic compounds from Emblicaofficinalis L. in rat. Inflammopharmacology, 19 (6) : 327-334.

  • Muthuraman, M., Hellriegel, H., Hoogenboom, N., Anwar, A.R., Mideksa, K.G., Krause, H., Schnitzler, A., Deuschl, G. and Raethjen, J. (2014). Beamformer source analysis and connectivity on concurrent EEG and MEG Data during voluntary movements. PLoS One, 9 (3): e91441, 10.1371/journal.pone.0091441.

  • Ng, K., Gu, C., Zhang, H. and Putri, C. Y. (2015). Evaluation of α-amylase and -glucosidase inhibitory activity of flavonoids. Internat. J. Food & Nutr. Sci., 2(2): 174-179.

  • Nivetha, G., Vishnupriya, V. and Gayathri, R. (2016). Comparative evaluation of anti-diabetic activity of lemon grass oil and Tulasi Oil. Int. J. Pharm. Sci. Rev. Res., 39 (1) : 221-225.

  • Parengkuan, L., Yagi, M., Matsushima, M., Ogura, M., Hamada, U. and Yonei, Y. (2013). Anti-glycation activity of various fruits. Anti-Aging Med., 10 : 70 -76.

  • Perera, H. K. I. and Wijetunge, D. C. R. (2015). Strong protein glycation inhibitory potential of clove and coriander. British J. Pharmaceutical Res., 6 (5) : 306-312.

  • Picot, C., Subratty, A. H. and Mahomoodally, M. F. (2014). Inhibitory potential of five traditionally used native antidiabetic medicinal plants on α-amylase, glucosidase, glucose entrapment and amylolysis kinetics in vitro. Adv. Pharmacological Sci., http://dx.doi.org/ 10.1155/2014/739834.

  • Poongunran, J., Perera, H. K.I., Fernando, W. I. T., Jayasinghe, L. and Sivakanesan, R. (2015). Alpha-glucosidase and alpha-amylase inhibitory activities of nine Sri Lankan antidiabetic plants. British J. Pharmaceutical Res., 7(5) : 365-374.

  • Rang, H. P. (2003). Anthelmintic drugs. In: Rang, H.P., Dale, M.M., Ritter, J.M. and Moore, P.K., Book Review: Pharmacology, 5th Ed., Churchill Livingstone, Edinburgh.

  • Rasouli, H., Hosseini-Ghazvini, S.M.B., Adibi, H. and Khodarahmi, R. (2017). Differential α-amylase/glucosidase inhibitory activities of plant-derived phenolic compounds: a virtual screening perspective for the treatment of obesity and diabetes. Food & Function, 8 (5) : 1942-1954.

  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cationdecolorization assay. Free Radical Biology &Medicine, 26(9-10) : 1231-1237.

  • Roy, A., Geetha, R. V., Lakshmi, T. and Nallanayagam, M. (2011). Edible fruits—nature’s gift for diabetic patients a comprehensive review. Internat. J. Pharmaceutical Sci. Rev. & Res., 9(2) : 170-180.

  • Saikia, S., Mahnot, N.K. and Mahanta, C.L. (2016). Phytochemical content and antioxidant activities of thirteen fruits of Assam, India. Food Bioscience, 13 : 1520.

  • Salehi, P., Asghari, B., Esmaeili, M. A., Dehghan, H. and Ghazi, I. (2013). -glucosidase and α-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes. J. Medicinal Plants Res., 7(6): 257-266.

  • Singleton, V. L. and Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American J. Enol. & Viticult., 16(3): 144-158.

  • Smruthi, G., Mahadevan, V., Vadivel, V. and Brindha, P. (2016). Docking studies on antidiabetic molecular targets of phytochemical compounds of [Syzygium cumini (L.) Skeels]. Asian J. Pharmaceutical & Clinical Research, 9 (3) : 287-293.

  • Sompong, W., Muangngam, N., Kongpatpharnich, A., Manacharoenlarp, C., Amorworasin, C., Suantawee, T. and Adisakwattana, S. (2016). The inhibitory activity of herbal medicines on the keys enzymes and steps related to carbohydrate and lipid digestion.BMCComplementary &Alternative Medicine, 16(1) : 439.

  • Srinivasan, P., Vijayakumar, S., Kothandaraman, S. and Palani, M. (2018). Anti-diabetic activity of quercetin extracted from Phyllanthusemblica L. fruit: In silico and in vivo approaches. J. Pharmaceutical Analysis, 8 (2) : 109-118.

  • Tadera, K., Minami, Y., Takamatsu, K. and Matsuoka, T. (2006). Inhibition of -glucosidase and α-amylase by flavonoids. J. Nutr. Sci. Vitaminol., 52(2) : 149-153.

  • Tupe, R. S., Sankhe, N. M., Shaikh, S. A., Phatak, D. V., Parikh, J. U., Khaire, A. A. and Kemse, N. G. (2015). Aqueous extract of some indigenous medicinal plants inhibits glycation at multiple stages and protects erythrocytes from oxidative damage–an in vitro study. J. Food Sci. & Technol,, 52(4) : 1911-1923.

  • Wang, S. H., Chang, J. C., Pokkaew, R., Lee, J. F. and Chiou, R. Y. Y. (2011). Modified fast procedure for the detection and screening of antiglycative phytochemicals. J. Agric. &Food Chem., 59(13) : 6906-6912.

  • Wang, T., Li, X., Zhou, B., Li, H., Zeng, J. and Gao, W. (2015). Anti-diabetic activity in type 2 diabetic mice and glucosidase inhibitory, antioxidant and anti-inflammatory potential of chemically profiled pear peel and pulp extracts (Pyrus spp.). J. Functional Foods, 13 : 276-288.

  • Woodman, O.L., Meeker, W.F. and Boujaoude, M. (2005). Vasorelaxant and antioxidant activity of flavonols and flavones: structure-activity relationships. J. Cardiovascular Pharmacol., 46(3) : 302-309.

  • Wu, C. H., Yeh, C. T., Shih, P. H. and Yen, G. C. (2010). Dietary phenolic acids attenuate multiple stages of protein glycation and high glucose stimulated proinflammatory IL -1β activation by interfering with chromatin remodeling and transcription in monocytes. Molecular Nutr. &Food Res., 54(S2) : S127-S140.

  • Xiao, J., Ni, X., Kai, G. and Chen, X. (2013). A review on structure–activity relationship of dietary polyphenols inhibiting α-amylase. Critical Rev. Food Sci. & Nutr., 53(5) : 497-506.

  • Yang, S. Y. (2010). Pharmacophore modeling and applications in drug discovery: challenges and recent advances. Drug Discovery Today, 15(11-12) : 444-450.

  • Yeh, W. J., Hsia, S. M., Lee, W. H. and Wu, C. H. (2017). Polyphenols with antiglycation activity and mechanisms of action: A review of recent findings. J. Food & Drug Analy., 25(1) : 84-92.

  • Zhishen, J., Mengcheng, T. and Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64 (4) : 555-559.

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  • Analysis of Edible Fruits against Glycolytic Enzymes and Glycation:In vitro Approaches with in silico Validation

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Authors

Anu Mishra
Laboratory of Foods and Nutrition, Post Graduate Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Anand (Gujarat), India
Hetalkumar Panchal
UGC-Centre of Advanced Study, Post Graduate Department of Biosciences, Sardar Patel University, Satellite Campus, Bakrol, Anand (Gujarat), India
V. H. Patel
Department of Home Science, Sardar Patel University, Vallabh Vidyanagar, Anand (Gujarat), India

Abstract


Diabetes is the most quotidian endocrine disorder and one of the fastest growing non-communicable diseases around the globe.Commonly consumed six fruits were investigated to determine their therapeutic potential to inhibitoxidation, key glycolytic enzymes and glycation activity which has relevance in the management of hyperglycemia and type II diabetes. The in vitro analysis revealed that amla fruit showed maximum total phenols and total antioxidant capacity among all the six fruits. Amla fruit exhibited potent inhibition for both alpha amylase and alpha glucosidase enzyme activity than the positive control acarbose. The IC50value of alpha amylase inhibition and alpha glucosidase inhibition in amla was found to be high among all the fruits. Further, amla fruit phenolic compound (gallic acid) confirmed better in silico enzyme inhibitory action with alpha glucosidase with a binding energy of -6.21kcal/mol than alpha amylase. In antiglycation activity amla and mango fruits showed potent inhibition. Pearson correlation results showed a strong correlation (p<0.01) between total phenol with flavonoids, total antioxidant capacity and antiglycation activities.The results obtained in this study showed that amla and mango had potent potential for the management of hyperglycemia, diabetes and the related condition of oxidative stress. Hence, these fruits can be prescribed to treat diabetes in safest way by incorporating them in natural medications.

Keywords


Fruits, Alpha Amylase, Alpha Glucosidase, Antiglycation, Antioxidant, In silico.

References