International Journal of Processing and Post harvest Technology

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Ultrasound Assisted Extraction Technique:Study of the Biological Properties of Allium stracheyi Baker


Affiliations
1 Department of Post Harvest Engineering and Technology, Aligarh Muslim University, Aligarh (U.P.), India
     

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An optimized Ultrasound Assisted extraction method for the extraction of Allium stracheyi Baker extract has been developed using polar (methanol) and non-polar (n-hexane) solvent at different concentrations (100%, 75%, 50% and 25%). The Bonferroni post hoc test revealed the presence of highest Total Phenolic Concentration (TPC), Total Flavonoid concentration (TFC) and DPPH per cent radical scavenging when using 25 per cent methanol at 50°C for 60 minutes. The temperature had significant effect on antioxidant activity (p<0.05) and non-significant effect on both TPC and TFC (p>0.05) whereas time showed significant effect for all (p<0.05). The antioxidant activity had positive correlation with the TPC and TFC content. The antibacterial data illustrated inhibition zones (mm) against E. coli for both hexane (100%) and methanol (100%) solvents. No inhibition zones were found against B. subtilis at the same solvent concentration. Temperature and time had no significant effect on % inhibition against the bacteria. No antibacterial activity was found against both the bacterial strains at 75 per cent, 50 per cent, 25 per cent hexane and methanol solvents. Hexane extracts showed larger inhibition zones than methanolic extracts.

Keywords

Ultrasound Assisted Extraction, Allium stracheyi Baker, TPC, TFC, Antibacterial Activity.
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  • Aksoy, L., Kolay, E., Aoylonu, Y., Aslan, Z. and Kargyoolu, M. (2013). Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsisturcica. Saudi J. Biol. Sci., 20(3) : 235-239.

  • Bezic, N, Skocibusic, M., Dunkic, V. and Radonic, A. (2003). Composition and antimicrobial activity of Achillea clavennae L. essential oil. Phytotherapy Res., 17(9) : 1037-1040.

  • Chang, C.C., Yang, M. H., Wen, H. M. and Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food & Drug Analysis, 10(3).

  • Danlami, J.M., Arsad, A., Zaini, A., Abbas, M. and Sulaiman, H. (2014). A comparative study of various oil extraction techniques from plants. Rev. Chemical Engg., 30(6) : 605-626.

  • Das, N.P. and Pereira, T.A. (1990). Effects of flavonoids on thermal autoxidation of palm oil: structure-activity relationships. J. American Oil Chemists’ Soc., 67(4) : 255-258.

  • Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S. and Ju, Y.H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J. Food & Drug Analysis, 22(3) : 296-302.

  • Ellner, P.D. and Neu, H.C. (1981). The inhibitory quotient: a method for interpreting minimum inhibitory concentration data. Jama, 246 (14) : 1575-1578.

  • Erkan, N., Ayranci, G. and Ayranci, E. (2008). Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chem., 110(1) : 76-82.

  • Gong, Y., Liu, X., He, W. H., Xu, H. G., Yuan, F. and Gao, Y.X. (2012). Investigation into the antioxidant activity and chemical composition of alcoholic extracts from defatted marigold (Tagetes erecta L.) residue. Fitoterapia, 83(3) : 481-489.

  • Guan, W., Li, S., Yan, R., Tang, S. and Quan, C. (2007). Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods. Food Chem., 101(4) : 1558-1564.

  • Jonathan, S.G. and Fasidi, I.O. (2003). Antimicrobial activities of two Nigerian edible macro-fungi-lycoperdonpusilum (Bat. Ex) and lycoperdongiganteum (Pers.). African J. Biomedical Res., 6(2).

  • Joshi, S. and Khan, M.A. (2016). An analysis of cold maceration based extraction technique for the extract of Allium stracheyi Baker. AFHABEC-2016Conference Proceedings.

  • Kamenetsky, R. and Rabinowitch, H.D. (2006). The genus Allium: A developmental and horticultural analysis.Hort. Rev., 32 : 329-378.

  • Kamiloglu, O.,Ercisli, S., Sengul, M., Toplu, C. and Serce, S. (2009). Total phenolics and antioxidant activity of jujube (Zizyphus jujube Mill.) genotypes selected from Turkey. African J. Biotechnol., 8(2).

  • Khanal, R.C., Howard, L.R. and Prior, R.L. (2010). Effect of heating on the stability of grape and blueberry pomace procyanidins and total anthocyanins. Food Res. Internat., 43(5) : 1464-1469.

  • Kilani, S., Sghaier, M.B., Limem, I., Bouhlel, I., Boubaker, J., Bhouri, W., Skandrani, I., Neffatti, A., Ammar, R.B., Dijoux-Franca, M.G. and Ghedira, K. (2008). In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperusrotundus. Bioresource Technol., 99(18) : 9004-9008.

  • Kumar, S., Pangti, J., Thakur, S., Upadhyay, N. and Jain, M. (2015). antioxidant and wound healing potential of standardized ethyl acetate fraction (as-3) of allium stracheyi in rats. IJBPAS, 4(12): 6467-6480.

  • Luque-Garcya, J.L. and De Castro, M.L. (2003). Ultrasound: a powerful tool for leaching. TrAC Trends in Analytical Chemistry, 22(1) : 41-47.

  • Mann, C.M. and Markham, J.L. (1998). A new method for determining the minimum inhibitory concentration of essential oils. J. Appl. Microbiol., 84(4) : 538-544.

  • McDonald, S., Prenzler, P.D., Antolovich, M. and Robards, K. (2001). Phenolic content and antioxidant activity of olive extracts. Food Chem., 73(1) : 73-84.

  • Mukherjee, A. and Rajasekaran, C. (2010). In-vitro hemolytic activity of Allium stracheyi baker. J. Pharmacy Res., 3(5) : 1160-1162.

  • Rajha, H.N., El Darra, N., Hobaika, Z., Boussetta, N., Vorobiev, E., Maroun, R.G. and Louka, N. (2014). Extraction of total phenolic compounds, flavonoids, anthocyanins and tannins from grape byproducts by response surface methodology. Influence of solid-liquid ratio, particle size, time, temperature and solvent mixtures on the optimization process. Food & Nutrition Sci., 5(4), 397.

  • Ramos, L., Kristenson, E.M. and Brinkman, U.T. (2002). Current use of pressurised liquid extraction and subcritical water extraction in environmental analysis. J. Chromatography A, 975(1) : 3-29.

  • Ranjan, S., Jadon, V. S., Sharma, N., Singh, K., Parcha, V., Gupta, S. and Bhatt, J.P. (2010). Antiinflammatory and analgesic potential of leaf extract of Allium stracheyi. J. Appl. Sci. Res., 6 : 139-143.

  • Rice-Evans, C. A., Miller, N. J. and Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. & Medicine, 20(7) : 933-956.

  • Riesenman, P.J. and Nicholson, W.L. (2000). Role of the spore coat layers in Bacillus subtilis spore resistance to hydrogen peroxide, artificial UV-C, UV-B, and solar UV radiation. Appl. & Environ. Microbiol., 66(2) : 620-626.

  • Sharma, O.P. and Bhat, T.K. (2009). DPPH antioxidant assay revisited. Food Chem., 113(4) : 1202-1205.

  • Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J. Agric. & Food Chem., 40(6) : 945-948.

  • Spigno, G., Tramelli, L. and De Faveri, D.M. (2007). Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J. Food Engg., 81(1) : 200-208.

  • Tiwari, U., Adams, S.J., Krishnamurthy, K.V., Ravikumar, K. and Padma, V. (2014). Pharmacognostic Studies on Two Himalayan Species of Traditional Medicinal Value. Notulae Scientia Biologicae, 6(2) : 149.

  • Vilkhu, K., Mawson, R., Simons, L. and Bates, D. (2008). Applications and opportunities for ultrasound assisted extraction in the food industry - A review. Innovative Food Sci. & Emerging Technologies, 9(2) : 161-169.

  • Wang, X., Wu, Y., Chen, G., Yue, W., Liang, Q. and Wu, Q. (2013). Optimisation of ultrasound assisted extraction of phenolic compounds from Sparganiirhizoma with response surface methodology. Ultrasonicssono Chem., 20(3) : 846-854.

  • Wiegand, I., Hilpert, K. and Hancock, R.E. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3(2) : 163-175.

  • Wong-Paz, J.E., Muniz-Marquez, D.B., Aguilar-Zarate, P., Rodriguez-Herrera, R. and Aguilar, C. N. (2014). Microplate quantification of total phenolic content from plant extracts obtained by conventional and ultrasound methods. Phytochemical Analysis, 25(5) : 439-444.

  • Wu, C., Wang, F., Liu, J., Zou, Y. and Chen, X. (2015). A comparison of volatile fractions obtained from Loniceramacranthoides via different extraction processes: ultrasound, microwave, Soxhlet extraction, hydrodistillation, and cold maceration. Integrative Med. Res., 4(3) : 171-177.

  • Youssef, El, H., Nicolas, L., Catherine, N. and Richard G.M. (2012). Low cost process for phenolic compounds extraction from cabernet sauvignon grapes (Vitis vinifera L. cv. CABERNET SAUVIGNON). Optimization by response surface methodology. Food & Nutrition Sci., 2012.


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  • Ultrasound Assisted Extraction Technique:Study of the Biological Properties of Allium stracheyi Baker

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Authors

Sweta Joshi
Department of Post Harvest Engineering and Technology, Aligarh Muslim University, Aligarh (U.P.), India
Mohammad Ali Khan
Department of Post Harvest Engineering and Technology, Aligarh Muslim University, Aligarh (U.P.), India

Abstract


An optimized Ultrasound Assisted extraction method for the extraction of Allium stracheyi Baker extract has been developed using polar (methanol) and non-polar (n-hexane) solvent at different concentrations (100%, 75%, 50% and 25%). The Bonferroni post hoc test revealed the presence of highest Total Phenolic Concentration (TPC), Total Flavonoid concentration (TFC) and DPPH per cent radical scavenging when using 25 per cent methanol at 50°C for 60 minutes. The temperature had significant effect on antioxidant activity (p<0.05) and non-significant effect on both TPC and TFC (p>0.05) whereas time showed significant effect for all (p<0.05). The antioxidant activity had positive correlation with the TPC and TFC content. The antibacterial data illustrated inhibition zones (mm) against E. coli for both hexane (100%) and methanol (100%) solvents. No inhibition zones were found against B. subtilis at the same solvent concentration. Temperature and time had no significant effect on % inhibition against the bacteria. No antibacterial activity was found against both the bacterial strains at 75 per cent, 50 per cent, 25 per cent hexane and methanol solvents. Hexane extracts showed larger inhibition zones than methanolic extracts.

Keywords


Ultrasound Assisted Extraction, Allium stracheyi Baker, TPC, TFC, Antibacterial Activity.

References