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To explore in vitro models in examining the influence of testosterone (T) to platelet activation through endothelial cells in hyperglycemia condition. Disperse primary and monolayer secondary human umbilical vein endothelial cells (HUVEC) in high glucose (HG) medium were exposed to T. Maximal platelet aggregation in the presence of HUVEC was measured using turbidimetric method. Maximal platelet aggregation in the presence of disperse primary HUVEC exposed to 0, 1, 10, and 100 nM of T were 69.4%, 65.4%, 67.6%, and 62.05%, whereas of monolayer secondary HUVEC were 46.5%, 43.9%, 48.1%, and 37.45%, respectively. Maximal platelet aggregation was significantly lower (p = 0.0001) in disperse primary HUVEC than in monolayer secondary HUVEC. The two methods were moderately correlated (r = 0.06; p = 0.079) in terms of endothelial activation.Maximal platelet agregation in the presence of disperse primary HUVEC is higher than, but correlated to, of monolayer secondary HUVEC, which is exposed to T in HG medium.


Platelet Aggregation, Endothelial Cells, Testosterone, High Glucose, In Vitro.
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