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Vanathi, P.
- Production and Characterization of Partially Purified Chitosanase Enzyme of Bacillus cereus A4/B4 Isolated from Biowaste Soil Samples:Bioactive Chitooligosaccharide
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PDF Views:561
Authors
P. Vanathi
1,
S. Fernandez
1,
M. Shanmugapriya
1,
T. S. Riyamol
1,
V. Loganathan
1,
V. I. Hairul-Islam
2,
P. Premasudha
3,
R. Rajendran
4
Affiliations
1 Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, IN
2 Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, IN
3 Department of Nanotechnology, Bharathiar University, Coimbatore, IN
4 PSG College of Arts & Science, Coimbatore, IN
1 Department of Microbiology, Maharaja Co Education Arts & Science College, Perundurai, IN
2 Division of Microbiology and immunology, Pondicherry centre for biological sciences, Pondicherry, IN
3 Department of Nanotechnology, Bharathiar University, Coimbatore, IN
4 PSG College of Arts & Science, Coimbatore, IN
Source
FoodSci: Indian Journal of Research in Food Science and Nutrition, Vol 2, No 2 (2015), Pagination: 41-48Abstract
Microbial chitosanase has received the attention of producing chitooligosaccharides. In this study, Chitooligosaccharide was developed by chitosanase enzyme hydrolysis and evaluated for its bioactivity. Chitosan degrading Bacillus cereus was isolated from biowaste sample and its extracellular enzyme chitosanase was characterized. The chitosanase enzyme production was induced by chitosan substrate and the enzyme production reached the maximum in 72 hours. The optimum pH and temperature for the production was 6.5 and 30°C respectively. The culture medium with 1.0 to 1.5% of substrate level with xylose served as significantly for enzyme production. The molecular weight of the enzyme 43KDa was determined by SDS-PAGE. The chitooligosaccharide produced by chitosanase hydrolysis inhibited the growth of the pathogens and showed MIC50 as 0.2 μg/ml against all the test pathogens and has proved to be a potential antimicrobial agent against skin and other infections.Keywords
Bacillus Species, Bioproduction, Chitosanase, Chitooligosaccharides, Microbial Enzymes.Full Text
References
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- Preparation of Chitooligosaccharide Mesh Graft using Freeze Gelation Method:Wound Dressing against Diabetic Foot Ulcers Pathogens
Abstract Views :628 |
PDF Views:426
Authors
Affiliations
1 PG and Research Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, Namakkal – 637205, Tamil Nadu, IN
1 PG and Research Department of Biotechnology, Sengunthar Arts and Science College, Tiruchengode, Namakkal – 637205, Tamil Nadu, IN
Source
FoodSci: Indian Journal of Research in Food Science and Nutrition, Vol 5, No 2 (2018), Pagination: 52-57Abstract
The importance of chronic wound treatment is with the management of the wound bed providing a moist environment to enhance epithelialisation in the wound healing process. The demand for the development of effective wound dressing in a cost effective condition is increasing day by day. On these points in view the present study was focused on to the preparation of the polymeric graft dressings based on chitosan for safer and reliable wound care management. In this regard to prepare chitosan based wound dressing the enzymatic degradation of chitosan was undertaken. The partially purified enzyme sample was taken for the degradation of the chitosan for the preparation the bioactive chitooligosaccharide. COS:PVA:collagen graft was prepared with the aldehyde compound. The wound scratching assay and the antimicrobial activity of the prepared graft has proved its antimicrobial and biocompatibility of the prepared graft.Keywords
Antimicrobial Activity, Chitooligosaccharides, Chitosan, Freeze Gelation Method, Wound Scratch Assay.Full Text
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