Preparation of Chitooligosaccharide Mesh Graft using Freeze Gelation Method:Wound Dressing against Diabetic Foot Ulcers Pathogens

The importance of chronic wound treatment is with the management of the wound bed providing a moist environment to enhance epithelialisation in the wound healing process. The demand for the development of effective wound dressing in a cost effective condition is increasing day by day. On these points in view the present study was focused on to the preparation of the polymeric graft dressings based on chitosan for safer and reliable wound care management. In this regard to prepare chitosan based wound dressing the enzymatic degradation of chitosan was undertaken. The partially purified enzyme sample was taken for the degradation of the chitosan for the preparation the bioactive chitooligosaccharide. COS:PVA:collagen graft was prepared with the aldehyde compound. The wound scratching assay and the antimicrobial activity of the prepared graft has proved its antimicrobial and biocompatibility of the prepared graft.