The Indian Journal of Nutrition and Dietetics

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Maternal Methyl-Cycle Amino Acid Profile and Kinetics:Relation with Placental Growth


Affiliations
1 Division of Nutrition, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
2 Department of Pathology, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
3 Division of Epidemiology and Biostatistics Unit, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
4 Department of Obstetrics and Gynecology, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
     

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Maternal intake of quality protein regulates placental development and function thereby affecting fetal growth. Considering the prevalence of inadequate intakes of quality protein in Indian pregnant women, understanding the interplay between maternal supply of protein, its metabolism and fetoplacental growth becomes important. A secondary analysis of data from an open labelled-randomized intervention trial with 500 ml milk/day on south Indian pregnant women with marginally low vitamin B12 status, was performed to assess the relations between placental parameters and maternal trimester 3 methyl-cycle amino acid status as well as kinetics. This analysis was performed for 42 pregnancies from the trial where placentae had been collected and placental parameters had been measured. For these pregnancies, data on trimester 3 methionine, serine and glycine kinetics as well as plasma free amino acid concentrations were available. Protein intake and plasma citrulline concentrations were positively correlated at trimester 3 (ρ = 0.34, P = 0.027). Placental weight correlated positively with methyl-cycle specific amino acid concentrations [methionine (ρ = 0.32, P = 0.0388), serine (ρ = 0.49, P = 0.0009)], methionine kinetics [total methionine flux rates (ρ = 0.42, P = 0.006), RM (ρ = 0.45, P = 0.003), TS (ρ = 0.32, P = 0.046), TM (ρ = 0.45, P = 0.004)] and with birth weight (ρ = 0.57, P < 0.001). Findings from the current study indicate that maternal amino acid availability and more importantly, maternal methionine kinetics, positively influenced placental growth, likely mediated by key amino acids such as citrulline, which is known to regulate placental blood flow and function. As an appropriately functioning placenta is indispensable for fetal growth, these findings will form the basis for detailed mechanistic explorations into the placental regulation of maternal supply of amino acid to the fetus for designing effective intervention strategies towards optimizing fetomaternal health during and after pregnancy.

Keywords

Vitamin B12, Pregnancy, Glycine, Methionine, Serine, Methionine Kinetic, Amino Acids.
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  • Godfrey, K., Robinson, S., Barker, D.J., Osmond, C. and Cox, V. Maternal nutrition in early and late pregnancy in relation to placental and fetal growth. BMJ. 1996, 312, 410-414.

  • Moore, V.M., Davies, M.J., Willson, K.J., Worsley, A. and Robinson, J.S. Dietary composition of pregnant women is related to size of the baby at birth. J. Nutr., 2004, 134, 1820-1826.

  • Herrera, E. Metabolic adaptations in pregnancy and their implications for the availability of substrates to the fetus. Eur. J. Clin. Nutr., [Internet]. 2000, 54, 47-51. Available from: http://www.ncbi.nlm.nih.gov/pubmed/10805038

  • Hadden, D.R. and McLaughlin, C. Normal and abnormal maternal metabolism during pregnancy. Semin. Fetal Neonat. Med., [Internet]. 2009, 14, 66-71. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1744165X08001157

  • Duggleby, S.L. and Jackson, A.A. Relationship of maternal protein turnover and lean body mass during pregnancy and birth length. Clin, Sci., (Lond). 2001, 101, 65-72.

  • Kalhan, S.C. One-Carbon Metabolism, Fetal Growth and Long-Term Consequences. 2013. p. 127–38. Available from: http://www.karger.com?doi=10.1159/000348459

  • Dasarathy, J., Gruca, L.L., Bennett, C., Parimi, P.S., Duenas, C., Marczewski, S. and Fierro, J.L. Methionine metabolism in human pregnancy 1- 3. Am. J. Clin. Nutr., 2010.

  • Kalhan, S.C., Uppal, S.O., Moorman, J.L., Bennett, C., Gruca, L.L., Parimi. P.S., Dasarathy, S., Serre, D. and Hanson, R.W. Metabolic and genomic response to dietary isocaloric protein restriction in the rat. J. Biol. Chem., 2011, 286, 5266-5277.

  • Kalhan, S.C. One carbon metabolism in pregnancy: Impact on maternal, fetal and neonatal health. Mol. Cell Endocrinol., [Internet]. 2016, 435, 48-60. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0303720716302052

  • Belkacemi, L., Nelson, D.M., Desai, M. and Ross, M.G. Maternal under nutrition influences placental-fetal development. Biolreprod., 2010, 83, 325-331.

  • Campbell, D.M., Hall, M.H., Barker, D.J., Cross, J., Shiella, W. and Godfrey, K.M. Diet in pregnancy and the offspring’s blood pressure 40 years later. Br. J. Obstet. Gynaecol., 1996, 103, 273-280.

  • Duggan, C., Srinivasan, K., Thomas, T., Samuel, T., Rajendran, R., Muthayya, S., Finkelstein, J.L., Lukose, A., Fawzi, W., Allen, L.H., et al. Vitamin B-12 supplementation during pregnancy and early lactation increases maternal, breast milk, and infant measures of vitamin B-12 status. J. Nutr., 2014, 144, 758-764.

  • Badaloo, A., Hsu, J.W., Taylor-Bryan, C., Green, C., Reid, M., Forrester, T. and Jahoor, F. Dietary cysteine is used more efficiently by children with severe acute malnutrition with edema compared with those without edema. Am. J. Clin. Nutr., [Internet]. 2012, 95, 84-90. Available from: http://www.ajcn.org/cgi/doi/10.3945/ajcn.111.024323

  • Dwarkanath, P., Hsu, J.W., Tang, G.J., Anand, P., Thomas, T., Thomas, A., Sheela, C., Kurpad, A.V. and Jahoor, F. Energy and protein supplementation does not affect protein and amino acid kinetics or pregnancy outcomes in underweight Indian women. J. Nutr., [Internet]. 2016, 146, 218-226. Available from: http://jn.nutrition.org/cgi/doi/10.3945/jn.115.218776

  • Windelberg, A., Årseth, O., Kvalheim, G. and Ueland, P.M. Automated assay for the determination of methylmalonic acid, total homocysteine, and related amino acids in human serum or plasma by means of methylchloroformate derivatization and gas chromatography-mass spectrometry. Clin. Chem., 2005, 51, 2103-2109.

  • Devi, S., Thomas, T., Dwarkanath, P., Thomas, A., Sheela, C.N., Mukhopadhyay, A. and Kurpad, A.V. Effect of increased dietary protein on the plasma methyl-cycle amino acid profile and kinetics during pregnancy. Ind. J. Nutr. Diet., 2016, 53, 380-390.

  • Jourdan, M., Nair, K.S., Carter, R.E., Schimke, J., Ford, G.C., Marc, J., Aussel, C. and Cynober, L. Citrulline stimulates muscle protein synthesis in the post-absorptive state in healthy people fed a low-protein diet – A pilot study. Clin. Nutr., [Internet]. 2015, 34, 449-456. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0261561414001277

  • Dasarathy, J., Gruca, L.L., Bennett, C., Parimi, P.S., Duenas, C., Marczewski, S., Fierro, J.L. and Kalhan, S.C. Methionine metabolism in human pregnancy. Am. J. Clin. Nutr., [Internet]. 2010, 91, 357-365. Available from: http://www.ajcn.org/cgi/doi/10.3945/ajcn.2009.28457

  • Kwon, H. Maternal nutrient restriction reduces concentrations of amino acids and polyamines in ovine maternal and fetal plasma and fetal fluids. Biol. Reprod., [Internet]. 2004, 71, 901-908. Available from: http://www.biolreprod.org/cgi/doi/10.1095/biolreprod.104.029645

  • Bourdon, A., Parnet, P., Nowak, C., Tran, N.T., Winer, N. and Darmaun, D.L. Citrulline supplementation enhances fetal growth and protein synthesis in rats with intrauterine growth restriction. J. Nutr., [Internet]. 2016, 146, 532-541. Available from: http://jn.nutrition.org/cgi/doi/10.3945/jn.115.221267

  • Moinard, C. and Cynober, L. Citrulline: A new player in the control of nitrogen homeostasis. J. Nutr., [Internet]. 2007, 137, 1621-1625. Available from: http://www.ncbi.nlm.nih.gov/ pubmed/17513438

  • Tran, N.T., Amarger, V., Bourdon, A., Misbert, E., Grit, I., Winer, N. and Darmaun, D. Maternal citrulline supplementation enhances placental function and fetal growth in a rat model of IUGR: involvement of insulin-like growth factor 2 and angiogenic factors. J. Matern Fetal Neonatal Med., [Internet]. 2016, 1-6. Available from: http:// www.ncbi.nlm.nih.gov/pubmed/27572235

  • Davis, S.R. Tracer-derived total and folate-dependent homocysteine remethylation and synthesis rates in humans indicate that serine is the main one-carbon donor. AJP Endocrinol. Metab., [Internet]. 2003, 286, 272-279. Available from: http://ajpendo.physiology.org/cgi/ doi/10.1152/ajpendo.00351.2003

  • Rees, W.D., Hay, S.M., Buchan, V., Antipatis, C. and Palmer, R.M. The effects of maternal protein restriction on the growth of the rat fetus and its amino acid supply. Br. J. Nutr., [Internet]. 1999, 81, 243-250. Available from: http://www.ncbi.nlm.nih.gov/pubmed/ 10434851

  • Thame, M., Fletcher, H., Baker, T. and Jahoor, F. Comparing the in vivo glycine fluxes of adolescent girls and adult women during early and late pregnancy. Br. J. Nutr., [Internet]. 2010, 104, 498-502. Available from: http://www.journals.cambridge.org/ abstract_S0007114510000784

  • Hayward, C.E., Lean, S., Sibley, C.P., Jones, R.L., Wareing, M., Greenwood, S.L. and Dilworth, M.R. Placental adaptation: what can we learn from birthweight:placental weight ratio? Front. Physiol., [Internet]. 2016, 7, 28. Available from: http://journal.frontiersin.org/ Article/10.3389/fphys.2016.00028/abstract.


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  • Maternal Methyl-Cycle Amino Acid Profile and Kinetics:Relation with Placental Growth

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Authors

Sarita Devi
Division of Nutrition, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Julian Crasta
Department of Pathology, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Tinku Thomas
Division of Epidemiology and Biostatistics Unit, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Pratibha Dwarkanath
Division of Nutrition, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Annamma Thomas
Department of Obstetrics and Gynecology, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
C. N. Sheela
Department of Obstetrics and Gynecology, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Anura V. Kurpad
Division of Nutrition, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India
Arpita Mukhopadhyay
Division of Nutrition, St. John’s Medical College, St. John’s National Academy of Health Sciences, Bangalore-560 034, India

Abstract


Maternal intake of quality protein regulates placental development and function thereby affecting fetal growth. Considering the prevalence of inadequate intakes of quality protein in Indian pregnant women, understanding the interplay between maternal supply of protein, its metabolism and fetoplacental growth becomes important. A secondary analysis of data from an open labelled-randomized intervention trial with 500 ml milk/day on south Indian pregnant women with marginally low vitamin B12 status, was performed to assess the relations between placental parameters and maternal trimester 3 methyl-cycle amino acid status as well as kinetics. This analysis was performed for 42 pregnancies from the trial where placentae had been collected and placental parameters had been measured. For these pregnancies, data on trimester 3 methionine, serine and glycine kinetics as well as plasma free amino acid concentrations were available. Protein intake and plasma citrulline concentrations were positively correlated at trimester 3 (ρ = 0.34, P = 0.027). Placental weight correlated positively with methyl-cycle specific amino acid concentrations [methionine (ρ = 0.32, P = 0.0388), serine (ρ = 0.49, P = 0.0009)], methionine kinetics [total methionine flux rates (ρ = 0.42, P = 0.006), RM (ρ = 0.45, P = 0.003), TS (ρ = 0.32, P = 0.046), TM (ρ = 0.45, P = 0.004)] and with birth weight (ρ = 0.57, P < 0.001). Findings from the current study indicate that maternal amino acid availability and more importantly, maternal methionine kinetics, positively influenced placental growth, likely mediated by key amino acids such as citrulline, which is known to regulate placental blood flow and function. As an appropriately functioning placenta is indispensable for fetal growth, these findings will form the basis for detailed mechanistic explorations into the placental regulation of maternal supply of amino acid to the fetus for designing effective intervention strategies towards optimizing fetomaternal health during and after pregnancy.

Keywords


Vitamin B12, Pregnancy, Glycine, Methionine, Serine, Methionine Kinetic, Amino Acids.

References