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Evaluation of Antibacterial Effect against Pseudomonas aeruginosa and Bacillus subtilis Using Different Solvents of Neolamarckia cadamba Fruit Extracts


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1 Department of Botany, Ranchi University, Ranchi, Jharkhand - 834 001, India
     

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Neolamarckia cadamba is a commonly found tree in India and is known for its large green leaves and ball shaped flowers and fruits. Also tree is culturally and economically important plant of India and is traditionally used for preparation of herbal medicines because of its various pharmacological properties. Although bark and leaves are mostly utilized whereas fruits are somewhat lagging behind in terms of its medicinal usages. This present study deals with the antibacterial properties of Neolamarckia cadamba (ripe and unripe) fruits. Six different solvent extracts which includes acetone, benzene, water, DMSO, ethanol and methanol (mg/ml) were prepared for the screening of antibacterial properties of Neolamarckia cadamba fruits against gram negative bacteria Pseudomonas aeruginosa and gram positive bacteria Bacillus subtilis. It was observed that zone of inhibition was highest in ethanolic extract (14 mm) of ripe fruit against the Bacillus subtilis. Zone of inhibition was found lowest in acetonic extract (5 mm) of ripe fruits whereas no zone of inhibition was found in benzene and ethanolic extract of unripe fruits. Out of both fruits, ripe was found to be most effective against both strains whereas out of six solvents ethanolic and methanolic extracts were found to be most effective.


Keywords

Neolamarckia Cadamba, Fruits, Solvents, Antibacterial, Disc Diffusion, Ofloxacin.
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  • Evaluation of Antibacterial Effect against Pseudomonas aeruginosa and Bacillus subtilis Using Different Solvents of Neolamarckia cadamba Fruit Extracts

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Authors

Swati Shikha
Department of Botany, Ranchi University, Ranchi, Jharkhand - 834 001, India
Anil Kumar
Department of Botany, Ranchi University, Ranchi, Jharkhand - 834 001, India

Abstract


Neolamarckia cadamba is a commonly found tree in India and is known for its large green leaves and ball shaped flowers and fruits. Also tree is culturally and economically important plant of India and is traditionally used for preparation of herbal medicines because of its various pharmacological properties. Although bark and leaves are mostly utilized whereas fruits are somewhat lagging behind in terms of its medicinal usages. This present study deals with the antibacterial properties of Neolamarckia cadamba (ripe and unripe) fruits. Six different solvent extracts which includes acetone, benzene, water, DMSO, ethanol and methanol (mg/ml) were prepared for the screening of antibacterial properties of Neolamarckia cadamba fruits against gram negative bacteria Pseudomonas aeruginosa and gram positive bacteria Bacillus subtilis. It was observed that zone of inhibition was highest in ethanolic extract (14 mm) of ripe fruit against the Bacillus subtilis. Zone of inhibition was found lowest in acetonic extract (5 mm) of ripe fruits whereas no zone of inhibition was found in benzene and ethanolic extract of unripe fruits. Out of both fruits, ripe was found to be most effective against both strains whereas out of six solvents ethanolic and methanolic extracts were found to be most effective.


Keywords


Neolamarckia Cadamba, Fruits, Solvents, Antibacterial, Disc Diffusion, Ofloxacin.

References