The Indian Journal of Nutrition and Dietetics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Quantitative Determination of Vitamin E Isomers Using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography


Affiliations
1 Kaleesuwari Refinery Pvt. Ltd., Department of Research and Development, Mambakkam, Vengaivasal, Chennai - 600 126, India
     

   Subscribe/Renew Journal


Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

Keywords

Tocopherol, Antioxidant, GC, HPLC, HPTLC.
User
Notifications

  • Maria Laura Colombo, An update on vitamin E, tocopherol and tocotrienol, Molecules, 2010, 15, 2103–2113.

  • Ubaldi, A., Delbono, G., Fusari, A. and Serventi, P. Quick HPLC method to determine vitamin E concentration in cow’s milk. Ann. Fac. Medic. Vet. di Parma., 2005, 25, 101–110.

  • Kaludin, I. Unterschungen fiber den Tokopherolgehalt in Serum und Erythrozyten von Ratten vershiedenen Alters. Znt. Z. Vitaminforsch, 1963, 33, 434–442.

  • Bieri, J.G., Poukka, R.K.H. and Prival, E.L. Determination of α-tocopherol in erythrocytes by gas-liquid chromatography, J. Lipid. Res., 1970, 11, 118–123.

  • Herbert J. Kayden, Ching-Kuang Chow and Loring, K. Bjornson, Spectrophotometric method for determination of tocopherol in red blood cells, J. Lipid Res., 1973, 14, 533–540.

  • Chiou, A., Karathanos, V.T., Mylona, A., Salta, F.N., Preventi, F. and Andrikopoulos, N.K. Currants (Vitisvinifera L.) content of simple phenolic and antioxidant activity. Fd. Chem, 2007, 102, 516–522.

  • Maciej Rutkowski, Krzysztof Grzegorczyk, Modification of spectrophotometric methods for antioxidative vitamins, determination convenient in analytical practice. Acta Sci. Pol., Technol. Aliment, 2007, 6, 17–28.

  • Tilay, A., Bule, M., Kishenkumar, J. and Annapure, U. Preparation of ferulic acid from agricultural wastes: its improved extraction and purification. J. Agric. Fd. Chem., 2008, 56, 7644–7688.

  • Mabinya, L.V., Mafunga, T. and Brand, J.M. Determination of ferulic acid and related compounds by thin layer chromatography. Afr. J. Biotechnol., 2006, 5, 1271–1273.

  • Swarali, S., Hingse, Shraddha, B., Digole and Uday, S. Annapure, Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography. J. Anal. Sci. Tech., 2014, 5, 21.


Abstract Views: 360

PDF Views: 1




  • Quantitative Determination of Vitamin E Isomers Using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

Abstract Views: 360  |  PDF Views: 1

Authors

K. Hari Ramakrishnan
Kaleesuwari Refinery Pvt. Ltd., Department of Research and Development, Mambakkam, Vengaivasal, Chennai - 600 126, India
Janaky Ranjithkumar
Kaleesuwari Refinery Pvt. Ltd., Department of Research and Development, Mambakkam, Vengaivasal, Chennai - 600 126, India

Abstract


Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

Keywords


Tocopherol, Antioxidant, GC, HPLC, HPTLC.

References