Open Access
Subscription Access
Open Access
Subscription Access
Retention of Xanthophylls in Green Foliar Vegetables after Different Food Preparations
Subscribe/Renew Journal
Green leafy vegetables (GLV) are rich sources of micronutrients, which have many health benefits and have the potential to combat the problem of malnutrition. However, domestic processing and cooking alter the nutrient contents. To study the retention of xanthophylls after processing, ten different GLV which are commonly available and regularly consumed, were analysed for total carotenoids, lutein, zeaxanthin and violaxanthin in both raw and cooked forms. In the unprocessed GLV, the lutein content on dry basis ranged from 341 mg/kg in Murraya koenigii to 959 mg/kg in Spinacia oleracea while zeaxanthin ranged from traces in Coriandrum sativum and Rumex acetosa to 15.45 mg/kg in Basella alba. In the case of violaxanthin, while it was not detectable in Hibiscus cannabinus, Amaranthus viridis had 794 mg/kg. The total carotenoids, lutein, zeaxanthin and violaxanthin in the processed GLV varied based on the variety of leafy vegetable cooked and the method of cooking. Microwave cooking followed by steaming were the most recommended method, while deep frying drastically reduced the total carotenoids and xanthophyll content followed by sautéing with oil. Overall, different methods of cooking resulted in changes in the phytochemical composition which are due to various factors such as leaf matrix, cooking method, cooking time and temperature.
Keywords
Nutrient Retention, Xanthophylls, Green Leafy Vegetables, Bioactive Phytochemicals, Cooking.
User
Information
- Tanumihardjo, S.A. and Yang, Z. Carotenoids: Epidemiology of Health Effects. Encyclopedia of Human Nutrition. 2nd ed. Oxford: Elsevier Ltd., 2005, 339-345.
- Johnson, E.J. A possible role for lutein and zeaxanthin in cognitive function in the elderly. Am. J. Clin. Nutr., 2012, 96(5), 1161S-1165S.
- Pugliese, A., Loizzo, M.R., Tundis, R., O’Callaghan, Y., Galvin, K., Menichini, F. and O’Brien, N. The effect of domestic processing on the content and bioaccessibility of carotenoids from chili peppers (Capsicum species). Fd. Chem., 2013, 141(3), 2606-2613.
- De Sa, M.C. and Rodriguez-Amaya, D.B. Optimization of HPLC quantification of carotenoids in cooked green vegetables-Comparison of analytical and calculated data. J. Fd. Compost. Anal., 2004, 17(1), 37-51.
- Leskova, E., Kubikova, J., Kovacikova, E., Kosicka, M., Porubska, J. and Holcíkova, K. Vitamin losses: Retention during heat treatment and continual changes expressed by mathematical models. J. Fd. Compost. Anal., 2006, 19(4), 252-276.
- AOAC, Official Methods of Analysis of the AOAC 2006, 15th Edition, Association of Official Analytical Chemists, Washington DC.
- Zakaria, M., Simpson, K., Brown, P.R. and Krstulovic, A. Use of reversed-phase high-performance liquid chromatographic analysis for the determination of provitamin A carotenes in tomatoes. J. Chromatogr. A., 1979, 176 (1), 109-117.
- Craft, N.E. Chromatographic techniques for carotenoid separation in Current Protocols in Food Analytical Chemistry, Unit F2.3, John Wiley and Sons Inc., New Jersey, USA, 2001.
- Znidarcic, D., Ban, D. and Sircelj, H. Carotenoid and chlorophyll composition of commonly consumed leafy vegetables in Mediterranean countries. Fd.Chem., 2011, 129 (3), 1164-1168.
- Raju, M., Varakumar, S., Lakshminarayana, R., Krishnakantha, T.P. and Baskaran, V. Carotenoid composition and vitamin A activity of medicinally important green leafy vegetables. Fd. Chem., 2011, 101(4), 1598-1605.
- Perry, A., Rasmussen, H. and Johnson, E.J. Xanthophyll (lutein, zeaxanthin) content in fruits, vegetables and corn and egg products. J. Fd. Composit. Anal., 2009, 22(1), 9-15.
- Biehler, E., Kaulmann, A., Hoffmann, L., Krause, E. and Bohn, T. Dietary and host-related factors influencing carotenoid bioaccessibility from spinach (Spinacia oleracea). Fd. Chem., 2011, 125(4), 1328-1334.
- Lakshminarayana, R., Raju, M., Krishnakantha, T.P. and Baskaran, V. Determination of major carotenoids in a few Indian leafy vegetables by high-performance liquid chromatography. J. Agric. Fd. Chem., 2005, 53(8), 2838-2842.
- Kim, M., Lee, J. and Choe, E. Effects of the number of fryings on pigment stability in frying oil and fried dough containing spinach powder. J. Fd. Sci., 2003, 68(3), 866-869.
- Miglio, C., Chiavaro, E., Visconti, A., Fogliano, V. and Pellegrini, N. Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. J. Agric. Fd. Chem., 2007, 56 (1), 139-147.
- Speek, A.J., Speek-Saichua, S. and Schreurs, W.H. Total carotenoid and β-carotene contents of Thai vegetables and the effect of processing. Fd. Chem., 1988, 27(4), 245-257.
- Khachik, F., Goli, M.B., Beecher, G.R., Holden, J., Lusby, W.R., Tenorio, M.D. and Barrera, M.R. Effect of food preparation on qualitative and quantitative distribution of major carotenoid constituents of tomatoes and several green vegetables. J. Agric. Fd. Chem., 1992, 40(3), 390-398.
- Djuikwo, V.N.D., Ejoh, R.A., Gouado, I., Mbofung, C.M. and Tanumihardjo, S.A. Determination of major carotenoids in processed tropical leafy vegetables indigenous to Africa. Fd. Nutr. Sci., 2011, 2(08), 793.
- Baloch, A.K., Buckle, K.A. and Edwards, R.A. Effect of processing variables on the quality of dehydrated carrot. Int. J. Fd. Sci. Technol., 1977, 12(3), 295-307.
- Hart, D.J. and Scott, K.J. Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK. Fd. Chem., 1995, 54(1), 101-111.
- Zhang, D. and Hamauzu, Y. Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Fd. Chem., 2004, 88(4), 503-509.
- Turkmen, N., Sari, F. and Velioglu, Y.S. The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Fd. Chem., 2005, 93(4), 713-718.
- Podsedek, A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Fd. Sci. Technol., 2007, 40(1), 1-11.
- Chew, E.Y., Clemons, T., SanGiovanni, J.P., Danis, R., Domalpally, A., McBee, W. and Ferris, F.L. The Age-Related Eye Disease Study 2 (AREDS2): study design and baseline characteristics (AREDS2 report number 1). Ophthalmol., 2012, 119(11), 2282-2289.
Abstract Views: 368
PDF Views: 1