The Indian Journal of Nutrition and Dietetics

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Immunity and Overall Tissue Health of Stinging Catfish, Heteropneustes Fossilis (Bloch, 1794) in Curcumin Medium


Affiliations
1 Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh
2 Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh, India
3 Department of Chemistry, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh, India
     

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Alternative safe ingredients are being increasingly promoted for resilient and sustainable activity, as they are in demand to replace drugs in aqua feed. Curcumin is a multifunction feed additive having immunostimulant and growth-promoting properties. In the present study, curcumin was synthesized and its potency was tested in the water on fish immunity and vital tissue anatomy. Adult Heteropneustes fossilis was treated for a short period (1, 5 days) to a long period (21 days) alone, further, its post-treatment clearance effect (one week without curcumin after 21 days treatment) was studied. The results showed that a mild dose of curcumin (10 μg/mL) in the medium was able to improve fish health (gill, liver, kidney histotexture) and immunity (direct and indirect haematology parameters) within a short duration and progressed further in a duration-dependent manner. Among haematological indices, some of them increased while others decreased significantly after curcumin treatment. Among immunity parameters white blood corpuscles, myeloperoxidase activity increased and nitroblue tetrazolium registered a decrease significantly, but the one-week post-treatment effect suggested poor maintenance of curcumin induced positive response towards health and immunity. Overall, the addition of curcumin (10 μg/mL) improved the growth performance and health of Heteropneustes fossilis.

Keywords

Curcumin, Haematology, Heteropneustes Fossilis, Histology
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  • Singh, K. and Mishra, A. Effect of season on helminth parasitic prevalence, dominance, means intensity and abundance in some fresh water scaly fish. Trds. Biosci., 2012, 5, 25-30.

  • Singh, K. and Mishra, A. A comparative study on seasonal distribution of the helminth parasites communities of some catfishes. Int. J. Pharma. Biosci., 2013, 4, 19-30.

  • Giri, S.S., Sahoo, S.K., Sahu, A.K. and Meher, P.K. Effectof dietary protein level on growth, survival, feed utilization and body composition of hybrid Clarias catfish (Clarias batrachus x Clarias gariepinus).Anim. Feed Sci. Technol. 2003, 104, 169-178.

  • Suárez, H., A. de Francisco, L.H. Beirão, J.M. Block, A. Saccol e S. Pardo. Importância de ácidos graxos poliinsaturados presentes em peixes de cultivo e de ambiente natural para a nutrição humana. Boletim do Instituto de Pesca, 2002, 28, 101-110.

  • Solari, F.A. Variaciones en la composición proteica del músculo de Colossoma macropomun (Cuvier,1818) (Characiformes: Characidae), provenientes de criaderos durante su almacenamiento en frío. Trabajo de grado. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima. 2006, 64.

  • González, Á., A. Márquez, W. Senior y G. Martínez. Contenido de grasa y proteina en Pygocentrus cariba, Prochilodus mariae, Plagioscion squamosissimus, Piaractus brachypomus e Hypostomus plecostomus en una laguna de inundación del Orinoco medio. Revista Científica FCV - LUZ. 2009, 19, 15-21.

  • Wanten, G.L. and P.C. Calder. Immune modulation by parenteral lipid emulsions. Am. J. Clini. Nutr., 2007, 85, 1171-1184.

  • Kris-Etherton, P.M., W.S. Harris and L.J. Appel. American heart association scientific statement, fish consumption, fish oil, omega 3 fatty acid, and cardiovascular disease. Circulat., 2002, 106, 2747-2757.

  • Carrero, J.J., E. Martín, L. Baró, J. Fonollá, J. Jiménez, J.J. Boza y E. López. Efectos cardiovasculares de los ácidos grasos omega-3 y alternativas para incrementar u ingesta. Nutrición Hospitalaria, 2005, 20, 63-69.

  • Li, M.H., E.H. Robinson, C.S. Tucker, B.B. Manning and L. Khoo. Effects of dried algae Schizochytrium sp., a rich source of docosahexaenoic acid, on growth, fatty acid composition, and sensory quality of channel catfish Ictalurus punctatus. Aquaculture, 2009, 292, 232-236.

  • Flores, P. El pescado: Su importancia en la nutrición y la promoción de su consumo. FONAIAP Divulga 26 (Octubre-Diciembre). 1987, 14.

  • Ersoy, B. and A. Özeren. The effect of cooking methods on mineral and vitamin contents of African catfish. Fd. Chem., 2009, 115, 419-422.

  • Orban, E., T. Nevigato, G.D. Lena, M. Masci, I. Casini, L. Gambelli and R. Caproni. (2008). New trends in the seafood market. Sutchi catfish (Pangasius hypophthalmus) fillets from Vietnam: Nutritional quality and safety aspects. Fd. Chem., 2008, 110, 383-389.

  • Greenfield, H. and D.A. Southgate. Food composition data: Production, management and use. Second edition. FAO, Rome. 2003, 289.

  • Veggetti, A., F. Mascarello, P.A. Scapolo and A. Rowlerson. Hyperplastic and hypertrophic growth of lateral muscle in Dicentrarchus labrax (L.). An ultrastructural and morphometric study. Anat. Embryol., 1990, 182, 1-10.

  • Galal, A.A.A., Reda, R.M. and Mohammad, A.A.R. Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide. Fish. Shellfish Immunol., 2018, 77, 445-456.

  • Galal, A.A.A., Reda, R.M. and Mohamm ad, A.A.R. Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide. Fish. Shellfish Immunol., 2018, 77, 445-456.

  • Mishra, A. and Singh, A. Attenuation of chlorpyrifos induced toxicity in freshwater catfish, Heteropneustes fossilis. Biochem. Cellu. Arc., 2020, 20, 5065-5072.

  • Mishra, A. and Singh, A. Effect of curcumin, ascorbic acid against chlorpyrifos toxicity on histotexture and bimolecular content of fresh water catfish Heteropneustes fossilis. J. Experim. Zoolog., 2020, 23, 1043-1049.

  • Menon, L.G., Kuttan, R. and Kuttan, G. Anti-metastatic activity of curcumin and catechin. Cancer Letters., 1999, 141, 159-165.

  • Zhang, F., Altorki, N.K., Mestre, J.R. and Maiah, K.A.J. Dannenberg, Curcumin inhibits cyclooxygenase-2 transcription in bile acid- and phorbol ester-treated human gastrointestinal epithelial cells. Carcinogen., 1999, 20, 445-451.

  • Shim, J.S., Kim, J.H., Cho, H.Y., Yum, Y.N., Kim, S.H., Park, H.J., Shim, B.S., Choi, S.H. and Kwon, H.J. Irreversible inhibition of CD13/aminopeptidase N by the antiangiogenic agent curcumin. Chem. Biol., 2003, 10, 695-704.

  • Lawler, J.M., Garcia-Villatoro, E.L., Guzzoni, V., Hord, J.M., Botchlett, R., Holly, D., Lawler, M.S., Gomes, M.J., Ryan, P., Rodriguez, D., Kuczmarski, J.M., Fluckey, J.D. and Talcott, S. Effect of combined fish oil and curcumin on murine skeletal muscle morphology and stress response proteins during mechanical unloading. Nutr. Res., 2019, 65, 17-28.

  • Vaishya, R., Agarwal, A.K., Shah, A., Vijay, V. and Vaish, A. Current status of top 10 nutraceuticals used for knee osteoarthritis in India. J. Clin. Orthopaed. Traum., 2018, 9, 338-348.

  • Burgos-Aceves, M.A., Lionetti, L. and Faggio, C. Multidisciplinary haematology as prognostic device in environmental and xenobiotic stress-induced response in fish. Sci. Total Environ., 2019, 670, 1170-1183.

  • Llacuna, S., Gorriz, A., Riera, M. and Nadal, J. Effects of air pollution on hematological parameters in passerine birds. Arc. Environ. Contam. Toxicol., 1996, 31, 148-152.

  • Mishra, A., Mishra, N., Rawat, A. and Verma, S. Effect of an organophosphate insecticide, chlorpyriphos on mortality and haematology of freshwater butter catfish Ompok bimaculatus (Bloch, 1794). Pensee J., 2014, 76, 452-461.

  • Maceda-Veiga, A., Figuerola, J., Martínez-Silvestre, A., Viscor, G., Ferrari, N. and Pacheco, M. Inside the Redbox: Applications of haematology in wildlife monitoring and ecosystem health assessment. Sci. Total Environ., 2015, 514, 322-332.

  • Kumari, J. and Sahoo, P.K. Non-specific immune response of healthy and immunocompromised Asian catfish (Clarias batrachus) to several immunostimulants. Aquaculture., 2006, 255, 133-141.

  • Sahoo, P.K., Kumari, J. and Mishra, B.K. Non-specific immune responses in juveniles of Indian major carps. J. Appli. Ichthyol., 2005, 21, 151-155.

  • Hinton, D.E. and Lauren, J.C. Integrative histopathology approaches to detecting effects of environmental stress on fishes. Am. Fisheries Soc. Sympos., 1990, 8, 51-66.

  • Camargo, M.M.P. and Martinez, C.B.R. Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotrop. Ichthyol., 2007, 5, 327-336.

  • Mishra, A. and Devi, Y. Histopathological alterations in the brain (optic tectum) of the fresh water teleost Channa punctatus in response to acute and subchronic exposure to the pesticide chlorpyrifos. Acta Histochemica., 2014, 116, 176-181.

  • Devi, Y. and Mishra, A. Histopathological alterations in gill and liver anatomy of fresh water, air breathing fish Channa punctatus after pesticide hilban (chlorpyrifos) treatment. Adv. Biores., 2013, 4, 57-62.

  • Fanta, E., Rios, F.S., Romão, S., Vianna, A.C.C. and Freiberger, S. Histopathology of the fish Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and food. Ecotoxicol. Environmen. Safety., 2003, 54, 119-130.

  • Khan, M.N., Saiful Islam, A.K.M. and Hussain, M.G. Marginal analysis of culture of stinging catfish (Heteropneustes fossilis, Bloch): Effect of different stocking densities in earthen ponds. Pak. J. Biolog. Sci., 2003, 6, 666-670.

  • Quade, M.J. and Roth, J.A. A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Veterinary Immunol. Immunopathol., 1997, 58, 239-248.

  • Wang, Y.J., Pan, M.H., Cheng, A.L., Lin, L.I., Ho, Y.S., Hsieh, C.Y. and Lin, J.K. Stability of curcumin in buffer solutions and characterization of its degradation products. J. Pharma. Biomed. Anal., 1997, 15, 1867-1876.

  • Storka, A., Vcelar, B., Klickovic, U., Gouya, G., Weisshaar, S., Aschauer, S., Helson, L. and Wolzt, M. Effect of liposomal curcumin on red blood cells in vitro. Anticancer Res., 2013, 33, 3629-3634.

  • Wahlstrom, B. and Blennow, G. A study on the fate of curcumin in the rat. Acta Pharmacologica et Toxicologica., 1978, 43, 86-92.

  • Yonar, M.E., Yonar, S.M., İspir, Ü. and Ural, M.Ş. Effects of curcumin on haematological values, immunity, antioxidant status and resistance of rainbow trout (Oncorhynchus mykiss) against Aeromonas salmonicida subsp. Achromogenes. Fish and Shellfish Immunol., 2019, 89, 83-90.

  • Deng, S.L., Chen, W.F., Zhou, B., Yang, L. and Liu, Z.L. Protective effects of curcumin and its analogues against free radical-induced oxidative haemolysis of human red blood cells. Fd. Chem., 2006, 98, 112-119.

  • Abbas, W.T., Ibrahim, T.B.E., Elgendy, M.Y. and Zaher, M.F.A. Effect of curcumin on iron toxicity and bacterial infection in catfish (Clarias gariepinus). Pak. J. Biolog. Sci., 2019, 22, 510-517.

  • Antony, S., Kuttan, R. and Kuttan, G. Immunomodulatory activity of curcumin. The Journal of Immunological Investigations, 28, 291-303. Relevant genes of Japanese flounder, Paralichthys olivaceus. Comparative Biochemistry and Physiology-Part D: Genomics and Proteomics., 2019, 1, 115-121.

  • Bunn, H.F. and Forget, B.G. Hemoglobin-molecular, Genetic, and Clinical Aspects. Published by W.B. Saunders Comp., 1986, 690.

  • Dalmo, R.A., Ingebrigtsen, K. and Bøgwald, J. Non-specific defence mechanisms in fish, with particular reference to the reticuloendothelial system (RES). J. Fish Dis., 1997, 20, 241-273.

  • Jagetia, G.C. and Aggarwal, B.B. Spicing up of the immune system by curcumin. The J. Clin. Immunol., 2007, 27, 19-35.

  • Anderson, D.P. and Siwicki, A.K. Basic haematology and serology for health programs. In Sharil, M., Arthur, J.R. and Subasinghe, R.P. (Eds.), Diseases in Asian Aquaculture II. Manila, Philippines: Philippines Fish Health Section, Asian Fisheries Society., 1995185.

  • Park, B.H. The use and limitations of the nitroblue tetrazolium test as a diagnostic aid. The J. Pediat., 1971, 78, 376-378.

  • Baehner, R.L., Nathan, D.G. and Karnovsky, M.L. Correction of metabolic deficiencies in the leucocytes of patients with chronic granulomatous disease. The J. Clin. Invest., 1970, 49, 865-870.

  • Sidhu, G.S., Singh, A.K., Thaloor, D., Banaudha, K.K., Patnaik, G.K., Srimal, R.C. and Maheshwari, R.K. Enhancement of wound healing by curcumin in animals. Wound Repair Regeneration., 1998, 6, 167-177.

  • Collin, M., Patel, N.S., Dugo, L. and Thiemermann, C. Role of peroxisome proliferator-activated receptor-γ in the protection afforded by 15-deoxy Δ 12, 14 prostaglandin J2 against the multiple organ failure caused by endotoxin. Critical Care Med., 2004, 32, 826-831.

  • Abdelrahman, M., Sivarajah, A. and Thiemermann, C. Beneficial effects of PPAR-γ ligands in ischemia-reperfusion injury, inflammation and shock. Cardiovascular Res., 2005, 65, 772-781.

  • Cao, L., Ding, W., Du, J., Jia, R., Liu, Y., Zhao, C., Shen, Y. and Yin, G. Effects of curcumin on antioxidative activities and cytokine production in Jian carp (Cyprinus carpio var. Jian) with CCl4-induced liver damage. Fish. Shellfish Immunol., 2015, 43, 150-157.


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  • Immunity and Overall Tissue Health of Stinging Catfish, Heteropneustes Fossilis (Bloch, 1794) in Curcumin Medium

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Authors

Pooja Pandey
Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh
Abha Mishra
Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh, India
Jyoti Pandey
Department of Chemistry, Babasaheb Bhimrao Ambedkar University, Lucknow - 226 025, Uttar Pradesh, India

Abstract


Alternative safe ingredients are being increasingly promoted for resilient and sustainable activity, as they are in demand to replace drugs in aqua feed. Curcumin is a multifunction feed additive having immunostimulant and growth-promoting properties. In the present study, curcumin was synthesized and its potency was tested in the water on fish immunity and vital tissue anatomy. Adult Heteropneustes fossilis was treated for a short period (1, 5 days) to a long period (21 days) alone, further, its post-treatment clearance effect (one week without curcumin after 21 days treatment) was studied. The results showed that a mild dose of curcumin (10 μg/mL) in the medium was able to improve fish health (gill, liver, kidney histotexture) and immunity (direct and indirect haematology parameters) within a short duration and progressed further in a duration-dependent manner. Among haematological indices, some of them increased while others decreased significantly after curcumin treatment. Among immunity parameters white blood corpuscles, myeloperoxidase activity increased and nitroblue tetrazolium registered a decrease significantly, but the one-week post-treatment effect suggested poor maintenance of curcumin induced positive response towards health and immunity. Overall, the addition of curcumin (10 μg/mL) improved the growth performance and health of Heteropneustes fossilis.

Keywords


Curcumin, Haematology, Heteropneustes Fossilis, Histology

References