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A Spectroscopy and Chromatography Based Study on Mineral Analysis, Amino Acid and Fatty Acid Composition of Polistes olivaceus, an Edible Insect Consumed in North East India


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1 Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam, India
     

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The study was aimed to analyze the mineral content, amino acid and fatty acid composition of Polistes olivaceus larvae consumed by different aboriginal tribes of North East India. Although the edible insect larvae has high market value in Assam and adjoining states of North East India, the nutritional analysis on it has not been done till now. Mineral elements including potassium, sodium, calcium, magnesium, phosphorus, zinc, iron and copper were detected by Atomic Absorption Spectroscopy. Amino acid composition and fatty acid profile were analysed by high performance liquid chromatography and gas chromatography mass spectrometry respectively to ascertain its potentiality to be included in food based strategies concerning human health. Iron and copper were the most abundant minerals and potassium, sodium and phosphorus were present in substantial amounts. Nineteen amino acids including all essential amino acids (43.87%) except isoleucine were detected in the sample. All essential amino acids satisfied the recommended level (score>100) except leucine with a chemical score of 98%. The level of unsaturated fatty acids was 53.21% while the saturated fatty acids constituted 47.02% of the total fatty acids signifying its potentiality in nutrition and health. Presence of linolenic acid as the main constituent of polyunsaturated fatty acids greatly signifies its importance in human nutrition. Thus, Polistes olivaceus larvae may be exploited to provide high-quality diets among populations plagued by iron deficiency as well as poor supply of dietary proteins and polyunsaturated fatty acids.

Keywords

Polistes olivaceus, Minerals, Amino Acids, Fatty Acids, Nutrition.
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  • Banjo, A.D., Lawal, O.A. and Songonuga, E.A. The nutritional value of fourteen species of edible insects in SouthWestern Nigeria. Af. J. Biotechnol., 2006, 5, 298–301.
  • Bukkens, S.G.F. The nutritional value of edible insects. Ecol. Fd. Nutr., 1997, 36, 287–319.
  • Elemo, B.O., Elemo, G.N., Makinde, M.A. and Erukainure, O.L. Chemical evaluation of African palm weevil, Rhychophorus phoenicis, larvae as a food source. J. Insect Sci., 2011, 11, 1–6.
  • DeFoliart, G.R. Insects as Food: Why the Western Attitude is important, Annual Review Entomol., 1999, 44, 21–50.
  • Finke, M.D. Complete nutrient content of four species of feeder insects. Zoo. Biol., 2013, 32, 27–36.
  • FAO, The state of food insecurity in the world (2009), Economic and social development department. Food and Agriculture Organization, Rome. 2010.
  • FAO, Edible forest insects. Human bites back. Food and Agriculture Organization, Rome. 2013.
  • Kinyuru, J.N., Konyole, S.O., Roos, N., Onyango, C.A., Owino V.O., Owuor B.O. and Estambale, B.B. Nutrient composition of four species of winged termites consumed in western Kenya. J. Fd. Compos. Analy., 2013, 30, 120–124.
  • Narzari, S. and Sarmah, J. Proximate composition of wild edible insects consumed by the Bodo tribe of Assam, India. Int. J. Bioassays, 2015, 4, 4050–4054.
  • Omotoso, O.T. Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda (Westwood) (Lepidoptera: Saturniidae). J. Zhejiang University Sci., 2006, 7, 51–55.
  • FAO/WHO, (2001). Human Vitamin and Mineral Requirements. Food and Nutrition Division, FAO, Rome.
  • Chakravorty, J., Ghosh, S. and Meyer-Rochow, V.B. Comparative survey of entomophagy and entomotherapeutic practices in six tribes of eastern Arunachal Pradesh (India). J. Ethnobiol. Ethnomed., 2013, 9, 1–12.
  • Longvah, T., Mangthya, K. and Ramulu, P. Nutrient composition and protein quality evaluation of eri silkworm (Samia ricinii) prepupae and pupae. Fd. Chem., 2011, 128, 400–403.
  • Shantibala, T., Lokeshwari, R.K. and Debaraj, H. Nutritional and anti-nutritional composition of the five species of aquatic edible insects consumed in Manipur, India. J. Insect Sci., 2014, 14, 1–10.
  • Narzari, S. and Sarmah, J. A study on the prevalence of entomophagy among the Bodos of Assam. J. Entomol. Zoolog. Stud., 2015, 3, 315–320.
  • Puwastein, P., Siong, T.E., Craven, G., Feliciano, R.R. and Judpsrasong. Asean Manual of Food Analysis, (1st Eds.), Regional Centre of ASEAN network of Food Data System. Institute of Nutrition, Mahidol University, 2011, 41–60.
  • Shi, Z., Li, H., Li, Z., Hu, J. and Zhang, H. Pre-column dervitization RP-HPLC determination of amino acids in Asparagi Radix before and after hearing Process. IERI Proced., 2013, 5, 351–356.
  • FAO/WHO/UNU, Expert consultation, Energy and protein requirements. WHO Technical Report Series No. 724. World Health Organization, Geneva, Switzerland. 1985.
  • Bligh, E.G. and Dyer, W.J. A rapid method of total lipid extraction and purification. Canad. J. Biochem. Physiol., 1959, 37, 911–917.
  • AOAC, Fat (Total, Saturated and unsaturated) in foods. Hydrolytic extraction gas chromatographic method. AOAC Official Methods 996.06. Revised edition of (1996), Association of the Analytical Chemists, Inc, Virginia, USA. 2001. 1–40.
  • Dietary Reference Intakes (DRIs), Recommended intake for individuals, elements. Food and Nutrition Board, Institute of Medicine, National Academics. 2004.
  • US Department of Agriculture, Agricultural Research Service, Nutrient Data Laboratory, USDA National Nutrient Database for Standard Reference, release 28, Version: September 2015.
  • Kinyuru, J.N., Kenji, G.N., Muhoho, S.N. and Ayieko, M. Nutritional potential of longhorn grasshopper (Ruspolia differens) consumed in Siaya district, Kenya. J. Agri. Sci. Technol., 2010, 12, 32–46.
  • Chakraborty, J., Ghosh, S., Megu, K., Jung, C. and Meyer-Rochow, V.B. Nutritional and anti-nutritional composition of Oecophylla smaragdina (Hymenoptera: Formicidae) and Odontotermes sp. (Isoptera:Termitidae): Two preferred edible insects of Arunachal Pradesh, India, J. Asia-Pacific Entomol., 2016, 19, 711–720.
  • Oliveira, S.J.F., Passos De Carvalho, J., Bruno De Sousa, R.F.X. and Madalena Simao, M. The nutritional value of four species of insects consumed in Angola. Ecol. Fd. Nutr., 1976, 5, 91–97.
  • Ekpo, K.E. and Onigbinde, A.O. Characterization of lipids in winged reproductive’s of the termite Macroterms bellicosus. Pak. J. Nutr., 2007, 6, 247–251.
  • Oyarzun, S.E., Graham, J. and Eduardo, V. Nutrition of the tamandua: I. Nutrient composition of termites and stomach contents from wild tamanduas (Tamandua tetradactyla). Zoo. Biol., 1996, 15, 509–524.
  • Hongo, T.A. Micronutrient malnutrition in Kenya. Af. J. Fd. Agri. Nutr. Develop., 2003, 3, 1–11.
  • Christensen, D.L., Orech, F.O., Mungai, M.N., Larsen, T., Friss, H. and Aagaard Hansen, J. Entomophagy among the luo of Kenya: a potential mineral source? Int. J. Fd. Sci. Nutr., 2006, 57, 198–203.
  • Yhoungaree, J., Puwastien, P. and Attig, G.A. Edible insects in Thailand: An unconventional protein source? Ecol. Fd. Nutr., 1997, 36, 133–149.
  • Bukkens, S.G.F. Insects in the human diet: nutritional aspects. In: MG Paoletti, (Eds.), Ecological implications of minilivestocks; role of rodents, frogs, snails and insects for sustainable development, 2005, 545–577. Science Publishers, New Hampshire.
  • Williams, P. Nutritional composition of red meat. Nutr. Diet., 2007, 64, 113–119.
  • Siemianowska, E., Kosewska, A., Aljewicz, M., Skibniewska, K.A., Polak-Juszczak, L., Jarocki, A. and Jêdras, M. Larvae of mealworm (Tenebrio molitor L.) as European novel food. Agri. Sci., 2013, 4, 287–291.
  • Walker, S.P., Wachs, T.D. and Gardner, J.M. Child development: risk factors for adverse outcomes in developing countries. Lancet, 2007, 369, 145–157.
  • Muros, M.J.S., Barroso, F.G. and Agugliaro, F.M. Insect as a suitable source of food for animal feeding: a review. J. Cleaner Product, 2014, 65, 16–27.
  • Ozimek, L., Sauer, W.C., Kozikowski, V., Ryan, J.K., Jorgensen, H. and Jelen, P. Nutritive value of protein extracted from honey bees. J. Fd. Sci., 1985, 50, 1327–1329.
  • Hill, M.M. Food Guides - Their Development and Use. Supplemental Nutrition Assistance Program News (Nutr Prog News, US Department of Agrculture), Washington DC. 1970.
  • De Souza, R.J., Andrew, M. and Adriana, M. Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease and type 2 diabetes: systematic review and meta analysis of observational studies. The Bri. Med. J., 2015, 351, 1–16.
  • Temme, E.H., Mensink, R.P. and Hornstra, G. Comparison of the effects of diets enriched in lauric, palmitic, or oleic acids on serum lipids and lipoproteins in healthy women and men. Am. J. Clini. Nutr., 1996, 63, 897–903.
  • Paoletti, M.G., Buscardo, E., Vanderjagt, D.J., Pastuszyn, A., Pizzoferato, L., Huang, Y.S., Chung, L.T., Glew, R.H., Millson, M. and Cerda, H. Nutrient content of termites (Syntermes soldiers) consumed by Makirtare Amerindians of the Alto Orinoco of Venezuela. Ecol. Fd. Nutr., 2003, 42, 173–187.
  • Raksakantong, P., Meeso, N., Kubola, J. and Siriamornpun, S. Fatty acids and proximate composition of eight thai edible tricolors insects. Fd. Res. Int. 2010, 43, 350–355.
  • Hayes, K.C. Dietary fat and heart health: in search of the ideal fat. Asia Pacific J. Clini. Nutr. 2002, 11, 394–400.
  • Mann, J. Disease of the heart and circulation: the role of dietary factors in aetiology and management. In: J.S. Garrow and James, W.P.T. (Eds.), Human Nutrition and Dietetics (pp. 619–650). Churchill, Livingstone London. 1993.

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  • A Spectroscopy and Chromatography Based Study on Mineral Analysis, Amino Acid and Fatty Acid Composition of Polistes olivaceus, an Edible Insect Consumed in North East India

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Authors

Silistina Narzari
Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam, India
Jatin Sarmah
Department of Biotechnology, Bodoland University, Kokrajhar - 783 370, Assam, India

Abstract


The study was aimed to analyze the mineral content, amino acid and fatty acid composition of Polistes olivaceus larvae consumed by different aboriginal tribes of North East India. Although the edible insect larvae has high market value in Assam and adjoining states of North East India, the nutritional analysis on it has not been done till now. Mineral elements including potassium, sodium, calcium, magnesium, phosphorus, zinc, iron and copper were detected by Atomic Absorption Spectroscopy. Amino acid composition and fatty acid profile were analysed by high performance liquid chromatography and gas chromatography mass spectrometry respectively to ascertain its potentiality to be included in food based strategies concerning human health. Iron and copper were the most abundant minerals and potassium, sodium and phosphorus were present in substantial amounts. Nineteen amino acids including all essential amino acids (43.87%) except isoleucine were detected in the sample. All essential amino acids satisfied the recommended level (score>100) except leucine with a chemical score of 98%. The level of unsaturated fatty acids was 53.21% while the saturated fatty acids constituted 47.02% of the total fatty acids signifying its potentiality in nutrition and health. Presence of linolenic acid as the main constituent of polyunsaturated fatty acids greatly signifies its importance in human nutrition. Thus, Polistes olivaceus larvae may be exploited to provide high-quality diets among populations plagued by iron deficiency as well as poor supply of dietary proteins and polyunsaturated fatty acids.

Keywords


Polistes olivaceus, Minerals, Amino Acids, Fatty Acids, Nutrition.

References