The Indian Journal of Nutrition and Dietetics

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Priliminary Phytochemical Screening and Antimicrobial Study of Dry and Fresh Aqueous, Ethanolic and Chloroform Extracts of Rosa Indica Petals


Affiliations
1 School of Biosciences, Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore - 641 043, Tamil Nadu, India
     

   Subscribe/Renew Journal


Plants are a valuable source of a multitude of secondary metabolites which have been used as medicine since times. Most unarguable merit of using plants as a source of medicine is that they are low priced and readily available with very minimal side effects. The present study deals with the comparative analysis of dry and fresh aqueous, ethanolic and chloroform extracts of Rosa indica petals for its phytochemical screening. The results confirmed the presence of almost all tested secondary metabolites in aqueous and ethanolic extracts, whereas chloroform extract showed only the presence of saponins. Further study was proceeded only with dry and fresh aqueous and ethanolic extracts of Rosa indica petals for its antimicrobial activity against Gram negative bacteria including Escherichia coli (ATCC NO: 25922), Pseudomonas aeruginosa (ATCC NO: 27853) and gram positive bacteria including Staphylococcus aureus (ATCC NO: 43300), Enterococcus faecalis (ATCC NO: 29212) and Candida albicans (ATCC NO: 24433) which is an opportunistic pathogenic yeast. Both dry and fresh ethanolic extracts showed a comparatively higher zone of inhibition for all the microbes. Aqueous dry and fresh extracts showed zone of inhibition for Staphylococcus aureus and Enterococcus faecalis, where as none of the extracts showed antimicrobial activity against Candida albicans. Results from the above study suggest that the ethanolic extract of Rosa indica petal are an encouraging alternative to some of the antibacterial drugs. This in turn would potentially be used as natural source to treat various multi drug resistant bacterial strains.

Keywords

Rosa Indica, Aqueous, Ethanolic, Phytochemicals, Antimicrobial Activity
User
Notifications

  • Sharma, M.S. and Choudhary, P.R. Effect of fenugreek seeds powder (Trigonella foenum-graecum L.) on experimental induced hyperlipidemia in rabbits. J. Diet. Suppl., 2017, 14, 1-8.

  • Amin, M.R., Mostofa, M., Hoque, M.E. and Sayed, M.A. In vitro anthelmintic efficacy of some indigenous medicinal plants against gastrointestinal nematodes of cattle. J. Bangladesh Agric. Univ., 2009, 7, 57-61.

  • Bruce Alberts., Alexander Johnson., Julian Lewis., Martin Raff., Keith Roberts et al., Molecular Biology of the Cell. New York., Garland Science, 2002, 4th edition.

  • Aiysha Thompson., Dilruba Meah., Nadia Ahmed., Rebecca Conniff-Jenkins., Emma Chilesh, E., et al Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome. BMC Complement Altern. Med., 2013, 1, 338.

  • Crepet, W.L., Nixon, K.C. and Gandolfo, M.A. Fossil evidence and phylogeny: The age of major angiosperm clades based on mesofossil and macrofossil evidence from cretaceous deposits. Am. J. Bot., 2004, 91, 1666-1682 .

  • Judd, W., Campbell, C., Kellogg, E., Stevens, P. and Donoghue, M. Plant Systematics. A Phylogenetic Approach. Sinauer Associates, Sunderland, Mass, USA, 1999.

  • Farooq, A., Khan, M.A., Ali, A. and Riaz, A. Diversity of morphology and oil content of Rosa damascena landraces and related Rosa species from pakistan. Pak. J. Agric. Sci., 2011, 48, 177-183.

  • Hunt, S.R. The rose in pharmacy. Pharm. J., 1962, 189, 589-591.

  • Singh, V., Kaul, V.K., Singh, B and Sood, R.P. Damask rose (Rosa damascena Mill.):Cultivation and processing, in Supplement to Cultivation and Utilization of Aromatic Plants. RRL, Jammu Tawi, India, 1997, 195.

  • Laxmi Sowmya, K., Sandhya Deepika, D., Geetha, S. and Lakshmi Sri, M. Biochemical and antimicrobial analysis of Rose petals (Rosa indica). Eur. J. Pharm. Sci., 2017, 4, 637-640.

  • Debiyi, O.O. and Sofowora, F. Pytochemical screening of medical plants. Iloyidia, 1978, 3, 234-246.

  • Roopashree, T.S., Dang, R., Rani, R.H.S. and Narendra, C. Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. Int. J. Appl. Res. Nat. Prod., 2008, 1, 20-28.

  • Sofowora, A. Phytochemical Screening of Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria, 1993.

  • Trease, G.E and Evans, W.C. Phenols and phenolic glycosides, Textbook of Pharmacognosy. Balliese, Tindall and Co Publishers, 1989, London, UK, 12, 343-383.

  • Vander, B.D.A. and Vlietrick. Screening methods for antibacterial and antiviral agents from higher plants. In: Assay for Bioactivity. K. Hostiettman (eds.). Academic Press, London. 1999, 47-69.


Abstract Views: 166

PDF Views: 1




  • Priliminary Phytochemical Screening and Antimicrobial Study of Dry and Fresh Aqueous, Ethanolic and Chloroform Extracts of Rosa Indica Petals

Abstract Views: 166  |  PDF Views: 1

Authors

Nithya Sethumadhavan
School of Biosciences, Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore - 641 043, Tamil Nadu, India
N. Santhi
School of Biosciences, Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore - 641 043, Tamil Nadu, India

Abstract


Plants are a valuable source of a multitude of secondary metabolites which have been used as medicine since times. Most unarguable merit of using plants as a source of medicine is that they are low priced and readily available with very minimal side effects. The present study deals with the comparative analysis of dry and fresh aqueous, ethanolic and chloroform extracts of Rosa indica petals for its phytochemical screening. The results confirmed the presence of almost all tested secondary metabolites in aqueous and ethanolic extracts, whereas chloroform extract showed only the presence of saponins. Further study was proceeded only with dry and fresh aqueous and ethanolic extracts of Rosa indica petals for its antimicrobial activity against Gram negative bacteria including Escherichia coli (ATCC NO: 25922), Pseudomonas aeruginosa (ATCC NO: 27853) and gram positive bacteria including Staphylococcus aureus (ATCC NO: 43300), Enterococcus faecalis (ATCC NO: 29212) and Candida albicans (ATCC NO: 24433) which is an opportunistic pathogenic yeast. Both dry and fresh ethanolic extracts showed a comparatively higher zone of inhibition for all the microbes. Aqueous dry and fresh extracts showed zone of inhibition for Staphylococcus aureus and Enterococcus faecalis, where as none of the extracts showed antimicrobial activity against Candida albicans. Results from the above study suggest that the ethanolic extract of Rosa indica petal are an encouraging alternative to some of the antibacterial drugs. This in turn would potentially be used as natural source to treat various multi drug resistant bacterial strains.

Keywords


Rosa Indica, Aqueous, Ethanolic, Phytochemicals, Antimicrobial Activity

References