The Indian Journal of Nutrition and Dietetics

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Determination of Total Microbial Bioload and Pathogen Testing from Bakery Products


Affiliations
1 Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India
     

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Bakery products are the most widely consumed food on the planet. Microbiological spoilage is one of the most common reasons for bakery products having a short shelf life. This study reports on isolation of microorganisms from bakery products. The study aimed to prospect the isolation of bacteria, total colony count and pathogen testing were carried out nutrient agar media and specific media. Isolation of bacteria was done using nutrient agar medium, total colony count was prosecuted by colony counting method and pathogen testing was performed using specific media like mannitol salt agar, EMB agar, cetrimide agar and bismuth sulphite agar. Biochemical characterization was executed for the obtained pathogen. It was preceded by test like indol test, MRVP test, simmon’s citrate test, catalase test, gelatinase test and TSI test. After completion of these tests it was concluded the obtained microorganism was Salmonella species.

Keywords

Bakery Products, Specific Media, Pathogen Testing, Biochemical Characterization, Salmonella Species
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  • Geetha, M. and Saranraj, P. Microbial spoilage of bakery products and its control by preservatives. Int. J. Pharmac. Biol. Arch., 2011, 3, 38-48.

  • Berdahl, Donald, R. and Jill McKeague. Rosemary and Sage Extracts as Antioxidants for Food Preservation. Published in Handbook of Antioxidants for Food Preservation, 2015. https://doi.org/10.1016/B978-1-78242-089-7.00008-7.

  • Gulzar Ahmad Nayik, Ishrat Majid. and Varun Kumar. Bakery industry in India - Innovations, treads and challenges - Food and Beverage News, 2014.

  • Das, K.K., Sarkar, A. and Hossain, A. Isolation of pathogenic microorganisms and determination of their antibiotic resistance patterns collected from different bakery products of Dhaka city. Fd. Res., 2020, 4, 1312-1316.

  • Anita Tull. Food and nutrition (3rd ed.), Oxford University Press, 1997, 154.

  • Jill Tricket. The Prevention of Food Poisoning, 2001, 8.

  • Agte, V., Chiplonkar, S., Hinge, A., Mengale, S. and Tarwadi, K. Vitamin profile of cooked foods: how healthy is the practice of ready-to-ear foods. Int. J. Fd. Sci. Nutr., 2002, 53, 197-208.

  • Bartrina, A.J., Majem, S.L., Rodrigo, P.C. and Rubio, D.A. Food habits of students using school dining rooms in Spain Tell me how you eat study. AtencionPrimaria, 2004, 33, 131-139.

  • Bernardino, A., Chianti,G., Corchia, M., Errico, M.K., Gelmetti, C.,Lovable, B., Rossetti,S., Ruggerini, A., Vanelli, M. and Volta, E. Breakfast habits of 1,202 northern Italian children admitted to a summer sport school breakfast skipping is associated with overweight and obesity. Acta Bio-medica Atenei Parmensis., 2005, 76, 79-85.

  • Berghofer, L.K., Hocking, A.D., Jansson, E. and Miskelly, D. Microbiology of wheat and flour milling in Australia. Int. J. Fd. Microbiol., 2003, 85, 137-149.

  • McLeod, S.A. Qualitative vs quantitative research. Simply Psychology, 2019, 2-14.

  • Dodd, C.E.R., Thompson, J.M. and Waites, W.M. Detection of rope spoilage in bread caused by Bacillus spp. J. Appl. Microbiol., 1998, 85, 481-486.

  • Adetuyi, F.C. and Ogundare, A.O. Studies on the microbial population of bread baked with wheat from south Western Nigeria. J. Fd. Agric. Environ., 2003, 2, 145-150.

  • Daifas, D.P., El- Khoury, A., El-Khoury, W., Koukoutsis. and Smith, J.P. Shelf life and safety concerns of bakery products-A review. Cri. Rev. Fd. Sci. Nutr., 2004, 44, 19-55.

  • Guynot, M.E., Marin, S., Ramos, A.J., Seto, L. and Sanchis, V. Screening for antifungal activity of some essential oils against common spoilage fungi of bakery products. Int. J. Fd. Sci. Technol., 2005, 11, 25-28.

  • Lapa, S.V., Pinchuk, I.V., Reva, O.N., Smirnov, V.V., Sorokulovo, I.B. and Urdaci, M.C. ‘Genetic diversity and involvement in bread spoilage of Bacillus strains isolated from flour and ropy bread. Letters. Appl. Microbiol., 2003, 37, 169-173.

  • Ahmad, A.A., Umoh, V.J. and Yusuf, I.Z. Occurrence and survival of Enterotoxigenic Bacillus cereus from some Nigeria flour based foods. Fd. Cont., 1992, 3, 149-152.

  • Allan, C., Von Holy, A. Current perspectives on rope in bread. Proceedings of the Second National Bakery Symposium. Int. Biodeterior. Biodegrad., 1990, 32, 119-125.


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  • Determination of Total Microbial Bioload and Pathogen Testing from Bakery Products

Abstract Views: 161  |  PDF Views: 1

Authors

V. Manivasagan
Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India
V. Archana
Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India
D. Dhanalakshmi
Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India
S. Shruthishankari
Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India
S. Vaishnavi
Department of Biotechnology, Adhiyamaan College of Engineering (Autonomous), Hosur - 635 130, Anna University, Chennai, Tamil Nadu, India

Abstract


Bakery products are the most widely consumed food on the planet. Microbiological spoilage is one of the most common reasons for bakery products having a short shelf life. This study reports on isolation of microorganisms from bakery products. The study aimed to prospect the isolation of bacteria, total colony count and pathogen testing were carried out nutrient agar media and specific media. Isolation of bacteria was done using nutrient agar medium, total colony count was prosecuted by colony counting method and pathogen testing was performed using specific media like mannitol salt agar, EMB agar, cetrimide agar and bismuth sulphite agar. Biochemical characterization was executed for the obtained pathogen. It was preceded by test like indol test, MRVP test, simmon’s citrate test, catalase test, gelatinase test and TSI test. After completion of these tests it was concluded the obtained microorganism was Salmonella species.

Keywords


Bakery Products, Specific Media, Pathogen Testing, Biochemical Characterization, Salmonella Species

References